Platelets A Practical Approach

Name: Platelets A Practical Approach
Availability: OutOfStock
ISBN: 9780199635375

ISBN-10: 0199635374

ISBN-13: 9780199635375

Edition: 1996

Authors: Stephen P. Watson, Kalwant S. Authi

List price: $125.00

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Description:

Platelets are small blood cells that play a vital role in blood clotting. They are essential for wound healing, but their unwanted action can lead to ailments such as stroke and heart attack. In seventeen chapters from leading experts in platelet research this book describes state-of-the-art techniques for the experienced researcher, both in academia and industry. Methodology is also included that will enable the newcomer to perform routine experiments. Techniques covered range from basic procedures such as platelet preparation to the latest techniques such as monitoring calcium levels in single cells, protein tyrosine phosphorylation, and PCR.

Book details

List price: $125.00
Copyright year: 1996
Publisher: Oxford University Press
Publication date: 8/1/1996
Binding: Paperback
Pages: 396
Size: 6.14" wide x 9.21" long x 0.83" tall
Weight: 1.496



Preface


Acknowledgement



Platelet preparation and functional responses



Cellular model systems to study megakaryocyte differentiation



Techniques in the preparation of semi-permeable platelets and their uses



Use of fluorescent indicators to measure intracellular Ca2+ and other ions



Preparation of highly purified human platelet plasma and intracellular membranes using high voltage free flow electrophoresis and methods to study Ca2+ regulation



The use of flow-cytometry to study platelet activation



Identification, isolation, and characterization of platelet glycoproteins mediating platelet adhesion



Monoclonal antibodies to platelet cell surface antigens and their use



Measurement of protein phosphorylation, kinase activity, and G protein function in intact platelets and membrane preparations



Tyrosine phosphorylation



Study of proteins associated with the platelet cytoskeleton



Methods for measuring agonist-induced phosphlipid metabolism in intact human platelets



Cyclic nucleotides: measurement and function



Immunocytochemical and electron microscope studies of platelets



Clinical aspects of platelet function



Platelet procoagulent activity and its measurement



The study of platelet function in other species


List of suppliers


Index