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Fundamentals of Enzymology The Cell and Molecular Biology of Catalytic Proteins

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ISBN-10: 019850229X

ISBN-13: 9780198502296

Edition: 3rd 1999 (Revised)

Authors: Nicholas C. Price, Lewis Stevens

List price: $99.00
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Description:

Since the publication of the successful and popular second edition of Fundamentals of Enzymology in 1989 there has been a large increase in the knowledge of several aspects of enzymology, not least the rapid acceleration of structural characterization of enzymes and the development of the field of bioinformatics. This new edition places appropriate emphasis on the new knowledge and consolidates the strengths of the previous editions. As before, Fundamentals of Enzymology 3rd ed gives an all-round view of the field including enzyme purification and characterization, enzyme structure (including information on World Wide Web sites), enzyme kinetics, the mechanisms and control of enzyme action, enzyme folding, how enzymes act in vivo, enzyme synthesis and degradation, and also clinical and industrial applications of enzymology. Throughout the book, the integration of these themes is stressed.
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Book details

List price: $99.00
Edition: 3rd
Copyright year: 1999
Publisher: Oxford University Press, Incorporated
Publication date: 2/17/2000
Binding: Paperback
Pages: 496
Size: 7.50" wide x 9.75" long x 1.25" tall
Weight: 2.442
Language: English

A note on units
List of abbreviations
Introduction
Aims of this book
Historical aspects
Remarkable properties of enzymes as catalysts
Cofactors
Nomenclature and classification of enzymes
The contents of this book
References
The purification of enzymes
Introduction
Why isolate enzymes?
Objectives and strategy in enzyme purification
Choice of source
Methods of homogenization
Methods of separation
How to judge the success of a purification procedure
Examples of purification procedures
Conclusions from the examples of enzyme purification
References
The structure of enzymes
Introduction
The determination of M[subscript r]
The determination of amino-acid composition and primary structure
The determination of secondary and tertiary structure
The determination of quaternary structure
The unfolding and folding of enzymes
Concluding remarks
References
Appendix 3.1
References for Appendix 3.1
An introduction to enzyme kinetics
Outline of the chapter
How do we obtain kinetic data?
How do we analyse kinetic data?
Pre-steady-state kinetics
Concluding remarks
References
Appendix 4.1
Appendix 4.2
Appendix 4.3
References for Appendices
The mechanism of enzyme action
Introduction
Definition of the mechanism of an enzyme-catalysed reaction
Background to catalysis
Experimental approaches to the determination of enzyme mechanisms
Examples of enzyme mechanisms
Concluding comments on enzyme mechanisms
References
The control of enzyme activity
Introduction
Control of the activities of single enzymes
Control of metabolic pathways
Examples of control of metabolic pathways
Concluding remarks
References
Appendix 6.1
Enzymes in organized systems
Introduction
Organized enzyme systems
RNA polymerase from E. coli
The occurrence and isolation of multienzyme proteins
Phylogenetic distribution of multienzyme proteins
Properties of multienzyme proteins
Pyruvate dehydrogenase multienzyme complex and related systems
Glycine decarboxylase multienzyme complex
The tryptophan synthase multienzyme complex from E. coli
Carbamoyl phosphate synthase and the associated enzymes of the pyrimidine and arginine biosynthetic pathways in E. coli, fungi, and mammalian cells
Multienzyme polypeptides: fatty-acid synthase and the arom complex (AROM enzyme)
Enzymes involved in DNA synthesis
The glycogen particle
Conclusions
References
Enzymes in the cell
Introduction
Intracellular compartmentation
Compartmentation of metabolic pathways
Vectorial organization of enzymes associated with membranes
The concentrations of enzymes and substrates in vivo
Conclusions
References
Enzyme turnover
Introduction
Kinetics of enzyme turnover
Methods for measurement of rates of enzyme turnover
Results from measurements of rates of enzyme turnover
Possible correlations between the rates of turnover and the structure and function of enzymes
The mechanisms of protein degradation
The significance of enzyme turnover
Other processes in which intracellular proteolysis is important
Conclusions
References
Appendix 9.1
Clinical aspects of enzymology
Introduction
Determination of enzyme activities for clinical diagnosis
Clinical enzymology of liver disease
Clinical enzymology of heart disease
Other enzyme activities that become elevated in serum in disease
The detection and significance of enzyme deficiencies
Enzyme inhibitors and drug design
The use of enzymes to determine the concentrations of metabolites of clinical importance
Enzyme therapy
Conclusions
References
Enzyme technology
Introduction
Use of microorganisms in brewing and cheesemaking
Use of microorganisms in the production of organic chemicals
Use of isolated enzymes in industrial processes
Immobilized enzymes
References
Enzymes referred to in Chapters 1-11
Index