Three-Dimensional Electron Microscopy of Macromolecular Assemblies Visualization of Biological Molecules in Their Native State

ISBN-10: 0195182189

ISBN-13: 9780195182187

Edition: 2nd 2005

Authors: Joachim Frank

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Description:

Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the moleculesof greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number ofworkers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
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Book details

Edition: 2nd
Copyright year: 2005
Publisher: Oxford University Press, Incorporated
Publication date: 2/2/2006
Binding: Paperback
Pages: 432
Size: 6.00" wide x 9.25" long x 1.00" tall
Weight: 1.320
Language: English

Introduction
Electron Microscopy of Macromolecular Assemblies
Two-Dimensional Averaging Techniques
Multivariate Data Analysis and Classification of Images
Three-Dimensional Reconstruction
Interpretation of Three-Dimensional Images of Macromolecules
Some Important Definitions and Theorems
Profiles, Point-Spread Functions, and Effects of Commonly Used Low-Pass Filters
Bibliography of Methods
Bibliography of Structures
Special Journal Issues on Image Processing Techniques
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