Reverse Transcriptase PCR

Name: Reverse Transcriptase PCR
Availability: OutOfStock
ISBN: 9780131231184

ISBN-10: 0131231189

ISBN-13: 9780131231184

Edition: 1995

Authors: Larrick, Paul D. Siebert

List price: $189.95

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Book details

List price: $189.95
Copyright year: 1995
Publisher: CRC Press LLC
Publication date: 1/1/1995
Binding: Hardcover
Pages: 346
Size: 7.00" wide x 10.00" long x 0.75" tall
Weight: 1.430
Language: English



Preface



Introduction and overview




RT-PCR on single cell after patch clamp recording




Reducing false positives with RNA template-specific PCR (RS-PCR)




Detection and analysis of T cell receptor gene products by RT-PCR




RT in situ PCR for detection of viral and mRNAs




A combined RT-PCR/Southern hybridization strategy and its application to homeotic mRNA expression in circulating CD34[superscript +] pluripotent stem cells




Evaluation of absolute mRNA levels by polymerase chain reaction-aided transcript titration assay (PATTY)




Limiting dilution polymerase chain reaction




Quantitative measurement of relative gene expression in human tumors




Quantitative RT-PCR based on multiplex amplification curves




[alpha]1 and [alpha]2 type IV collagen mRNAs in adult mouse and human glomeruli: quantitation by competitive PCR




Ligation-independent cloning of PCR products




Anchor ligated cDNA libraries: a technique for generating a cDNA library for the immediate cloning of the 5 ends of mRNAs




Equalized cDNA libraries




cDNA selection with cosmids and YACs




Semi-quantitative reverse transcriptase-polymerase chain reaction for the detection of low abundance transcripts in human cells




Detection of p53 gene mutations by RT-PCR-SSCP




SSCP analysis of the PCR product of RB cDNA




The application of RT-PCR to other infectious diseases