Practical Guide to the Study of Calcium in Living Cells

Name: Practical Guide to the Study of Calcium in Living Cells
Price: 48.14 USD
Availability: InStock
ISBN: 9780125641418

ISBN-10: 0125641419

ISBN-13: 9780125641418

Edition: 1994

Authors: Leslie Wilson, Paul T. Matsudaira, Richard Nuccitelli

List price: $72.95

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Description:

APractical Guide to the Study of Calcium in Living Cellsdescribes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Key Features * Describes techniques for detection of [Ca 2+ ]I: Ca 2+ - sensitive microelectrodes * Fluorescent dyes * Luminescent proteins * Includes techniques for perturbing intracellular Ca 2+ * Covers detailed methodology plus…

Book details

List price: $72.95
Copyright year: 1994
Publisher: Elsevier Science & Technology
Publication date: 4/25/1994
Binding: Hardcover
Pages: 368
Size: 5.94" wide x 9.00" long x 1.00" tall
Weight: 2.2
Language: English



Ca<sup>2+</sup> Buffers and [Ca<sup>2+</sup>]I Perturbation Techniques


A Practical Guide to the Preparation of Ca<sup>2+</sup> Buffers



Photorelease Techniques for Raising or Lowering Intracellular Ca<sup>2+</sup>



Pharmacological Tools for Perturbing Intracellular Calcium Storage



Microelectrode Techniques for Measuring [Ca<sup>2+</sup>]I and Ca<sup>2+</sup> Fluxes


How to Make and to Use Calcium-Specific Mini- and Microelectrodes



The Vibrating Ca<sup>2+</sup>



Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca<sup>2+</sup> Influx and Efflux


IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels



Fluorescence Techniques for Imaging [Ca<sup>2+</sup>]I


Practical Aspects of Measuring [Ca<sup>2+</sup>] with Fluorescent Indicators



Rapid Simultaneous Estimation of Intracellular Calcium and pH



Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques



Confocal Imaging of Ca<sup>2+</sup> in Cells



Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes



Use of Aequorin for [Ca<sup>2+</sup>]I Imaging


Inexpensive Techniques for Measuring [Ca<sup>2</sup>+]I Changes Using a Photomultiplier Tube



Imaging [Ca<sup>2+</sup>]I with Aequorin Using a Photon Imaging Detector



Targeting Recombinant Aequorin to Specific Intracellular Organelles



Index