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Molecular Biology Techniques A Classroom Laboratory Manual

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ISBN-10: 0123855446

ISBN-13: 9780123855442

Edition: 3rd 2012

Authors: Heather B. Miller, D. Scott Witherow, Sue Carson

List price: $105.00
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Description:

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still…    
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Book details

List price: $105.00
Edition: 3rd
Copyright year: 2012
Publisher: Elsevier Science & Technology
Publication date: 12/16/2011
Binding: Paperback
Pages: 232
Size: 8.46" wide x 10.87" long x 0.50" tall
Weight: 1.694
Language: English

Dr. Heather Miller is an Assistant Professor of Biochemistry in the Chemistry Department at High Point University (High Point, NC). She graduated from Clarion University of Pennsylvania (Clarion, PA) with a B.S. in Molecular Biology/Biotechnology, and from Duke University (Durham, NC) with a Ph.D. in Molecular Genetics and Microbiology. She completed a teaching postdoctoral position in the Biotechnology Program at North Carolina State University (Raleigh, NC). Her area of scientific expertise is RNA biology. Her research focuses on HIV-1 gene expression and the coupling of transcription and splicing in humans. She has taught at the college level for nine years, and is engaged in the…    

Foreword
Acknowledgements
Note to Instructors
Instrumentation
Nomenclature
Introduction: Conceptual outline for experiments
Manipulation of DNA
Screening Transformants
Expression, Detection, and Purification Of Recombinant Proteins
Appendices: Equipment, Prep list, Making sense of orientation