Human Stem Cell Manual A Laboratory Guide

ISBN-10: 0123854733

ISBN-13: 9780123854735

Edition: 2nd 2012

Authors: Suzanne Peterson, Jeanne F. Loring

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This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.. Offers a comprehensive introduction to stem cell biology and culture for medical and biology researchers investigating diagnostics and treatments for various diseases. Presents a historical introduction, discussion of alternative methods, and common pitfalls for basic and advanced experimental strategies. Includes new chapters devoted to iPS cells and other alternative sources for generating human stem cells written by the scientists who made these breakthroughs
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Book details

List price: $99.95
Edition: 2nd
Copyright year: 2012
Publisher: Elsevier Science & Technology Books
Publication date: 8/27/2012
Binding: Comb Bound 
Pages: 656
Size: 8.50" wide x 10.00" long x 1.75" tall
Weight: 2.926
Language: English

Basic methods: Maintenance of stem cell cultures
Human Embryonic Stem Cell Culture
Feeder layers and feeder-free culture
Mouse Embryonic Fibroblast Feeder Cells
Cryopreservation of hESC
Basic methods: Characterization of stem cells
Classical Cytogenetics: Karyotyping
Spectral Karyotyping and Fluorescent in situ Hybridization
Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns
FACS/Cell sorting
Fluorescence Immunocytochemical Analysis of Stem Cells
Characterization of Stem Cells Using RT-PCR
Gene Expression Profiling of Stem Cells by Microarray
Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice
Generation of HESC-Derived Teratomas
Differentiation of stem cells
"Embryoid Body�Ǡ Formation and Neuroepithelia Differentiation
Motor Neuron and Dopamine Neuron Differentiation
Oligodendrocyte Differentiation from hESC
Genetic manipulation of stem cells
Genetic Manipulation of hESC: Lentivirus Vectors
Methods developed for mouse ES cells
Advanced methods
Derivation of human ES cells
Neural Stem Cell Culture
Transplantation of stem cells
In vitro fertilization
Functional characterization-neural cells
Practical issues in establishing a human ES cell laboratory
Setting Up a Facility for hESC Research
Essays on stem cell biology
Intellectual Property: Owning the Stem Cell
Ethical Concerns for Stem Cell Research
Guidelines for hESC Research Oversight (ESCRO) Committees
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