Chemokines, Part A

Name: Chemokines, Part A
Availability: OutOfStock
ISBN: 9780123749086

ISBN-10: 0123749085

ISBN-13: 9780123749086

Edition: 461st 2009

Authors: Tracy Handel, Damon Hamel, Damon Hamel, Damon Hamel

List price: $225.00

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Description:

The understanding of chemokines, the proteins that control the migration of cells, and their receptors, is critical to the study of causes and therapies for a wide range of human diseases and infections, including certain types of cancer, inflammatory diseases, HIV, and malaria. This volume, focusing on chemokines as potential targets for disease intervention, and its companion volume (Methods in Enzymology volume 462, focusing on chemokine structure and function, as well as signaling) provide a comprehensive overview and time-tested protocols in this field, making it an essential reference for researchers in the area. Gathers tried and tested methods and techniques from top players in the…

Book details

List price: $225.00
Edition: 461st
Copyright year: 2009
Publisher: Elsevier Science & Technology
Publication date: 7/8/2009
Binding: Hardcover
Pages: 400
Size: 6.00" wide x 9.25" long x 1.50" tall
Weight: 2.134
Language: English



Contributors


Preface


Volumes in Series



Chemokines and Receptors in Disease



Chemokines in Human Breast Tumor Cells: Modifying Their Expression Levels and Determining Their Effects on the Malignancy Phenotype




Introduction



Modifying Chemokine Expression in Breast Tumor Cells



Establishment of Primary Local Breast Tumors and Pulmonary Metastases


Acknowledgments


References



CCR5 Pharmacology Methodologies and Associated Applications




Introduction



CCR5 Signaling Assays and Application to Quantify and Characterize Ligand-Dependent Agonism, Antagonism, and Inverse Agonism



CCR5-Associated Ligand-Binding Assays



Surrogate In Vitro Antiviral Assays



CCR5 Site-Directed Mutagenesis and Ligand Docking Studies



Non-HIV Indications-Associated Studies, Human CCR5 Knock-In Mice


References



CXCR4 and Mobilization of Hematopoietic Precursors




Introduction



HSPC Mobilizing Agents that Target the CXCL12/CXCR4 Axis



Donor Selection for HSPC Mobilization



Flow Cytometric Enumeration of Mobilized HSPCs



Dosing and Kinetics of HSPC Mobilization by G-CSF and Plerixafor



Flow Cytometric Analysis of CXCR4 Expression on Human CD34<sup>+</sup> Subsets



Functional Characterization of Mobilized HSPCs



Concluding Remarks


Acknowledgment


References



Double-Label Nonradioactive In Situ Hybridization for the Analysis of Chemokine Receptor Expression in the Central Nervous System




Introduction



Basic Protocol for ISH (Using Digoxygenin-Labeled Probe)



Comments


References



Expression of Chemokines and Chemokine Receptors in Human Colon Cancer




Introduction



Materials and Methods



Results



Discussion


Acknowledgments


References



Chemokine Related Proteins from Pathogens



Kaposi's Sarcoma Virally Encoded, G-Protein-Coupled Receptor: A Paradigm for Paracrine Transformation




Introduction



Cloning of vGPCR



Assaying vGPCR Transforming Activity In Vitro



vGPCR Transforming Activity In Vivo Using Xenograft Systems



In Vivo Targeted Infection Using the TVA-RCAS System



vGPCR-Induced Paracrine Transformation



Characterization of vGPCR-Induced Molecular Signaling



Evaluation of Activation of Second-Messenger-Generating Systems



Activation of Signal-Transducing Protein Kinases and Small GTPases



Akt Kinase Assay



vGPCR Stimulated Activation of Rac1-Pulldown Assays



Western Blotting Using Phospho-Specific Antibodies



Activation of Transcription Factors



Global Changes in Gene Expression: Microarray Analysis



NFkB Luciferase Assays



NFkB Binding Assays



Nuclear Translocation of NFkB


Acknowledgments


References



Pharmacological and Biochemical Characterization of Human Cytomegalovirus-Encoded G Protein-Coupled Receptors




Introduction



Virally Encoded GPCR Engineering



vGPCR Expression, Trafficking, and Radioligand Binding



vGPCR-Induced Signal Transduction



vGPCR-Induced Oncogenesis



Generation of Recombinant HCMV Strains by Markerless Bacterial Artificial Chromosome Mutagenesis



Conclusions


Acknowledgments


References



Identification and Characterization of Virus-Encoded Chemokine Binding Proteins




Introduction



Methods for Studying Chemokine-Binding Proteins


Acknowledgments


References



The Chemokine-Binding Protein M3 as a Tool to Understand the Chemokine Network In Vivo




Introduction



Generation of Transgenic Mice Expressing M3 in Insulin-Producing b Cells



M3 Expression in Islets of Langerhans Blocks CCL2-, CCL21-, and CXCL13-Induced Migration of Cells to Islets



M3 Expression in b Cells Blocks Cellular Infiltration and Prevents Diabetes Development



Generation of a Conditional Transgenic System for Expression of M3



Concluding Remarks


References



M-T7: Measuring Chemokine-Modulating Activity




Introduction



Protein Expression



Quantifying the Effects of M-T7 In Vitro and Ex Vivo



Quantifying the Effects of M-T7 on Vascular Inflammatory Responses in Rodent Vascular Transplant Models



Preclinical Toxicity Testing


References



Atypical and Novel Chemoattractants and Receptors



Role of the Chemokine Scavenger Receptor D6 in Balancing Inflammation and Immune Activation




Introduction



Methods



Results



Discussion


References



Structure-Function Dissection of D6, an Atypical Scavenger Receptor




Introduction


Acknowledgments


References



Modeling Small Molecule-Compound Binding to G-Protein-Coupled Receptors




Introduction



Similarity and Differences in the Crystal Structures of Class-A GPCRs Solved to Date



GPCR Modeling Methods



Computational Methods for Receptor Flexibility and Ligand-Induced Conformational Changes in GPCRs



Validation of GPCR-Ligand Models



Conclusions


References



Elucidation of Chemerin and Chemokine-Like Receptor-1 Function in Adipocytes by Adenoviral-Mediated shRNA Knockdown of Gene Expression




Introduction



The 3T3-L1 Cell Model for Adipogenesis and Adipocyte Metabolism



RNA Interference



Methods for Adenoviral shRNA Knockdown of Chemerin and CMKLR1 in 3T3-L1 Cells



Concluding Remarks


Acknowledgments


References



Chemokine Signaling



Characterization of Chemokine Receptor CXCR2 Interacting Proteins Using a Proteomics Approach to Define the CXCR2 "Chemosynapse"




Introduction



Validation of the Interaction of Novel Proteins with CXCR2



Mutational Analysis of Residues at Interactive Interface of CXCR2 and CXCR2-Binding Proteins



Radioactive Phosphorylation of CXCR2 Interacting Proteins



Chemotaxis Assay



Conclusions


Acknowledgments


References



Phosphoproteomic Analysis of Chemokine Signaling Networks




Introduction



Methods



Summary


Acknowledgments


References



Monitoring NF-kB Mediated Chemokine Transcription in Tumorigenesis




Introduction



Development of NF-kB Reporter Model for Tumors



Bioluminescent Imaging of Intratumor Signaling of Anesthetized Mice



Cell-Based Assays for Kinase and Transcriptional Activity In Vitro



Peripheral Spying of Intratumoral Signaling of Conscious Mice


Acknowledgments


References



Analysis of Chemokine Receptor Endocytosis and Intracellular Trafficking




Introduction



Receptor Detection



Cells



Monitoring Receptor Endocytosis



Monitoring Receptor Recycling



Monitoring Receptor Degradation



Electron Microscopy Analysis of Receptor Internalization


Acknowledgments


References



Measuring the Proximity of T-Lymphocyte CXCR4 and TCR by Fluorescence Resonance Energy Transfer (FRET)




Introduction



Assaying CXCR4-TCR Proximity via the PE/APC mAb FRET Approach



Assaying CXCR4-TCR Proximity via the CFP/YFP Fusion Protein Approach



Concluding Remarks


References



Expression of CXCR4, a G-Protein-Coupled Receptor for CXCL12 in Yeast: Identification of New-Generation Inverse Agonists




Introduction



Methods and Discussion



Summary


References



Ubiquitination of Chemokine Receptors




Introduction



Cell Culture and Transfections



Agonist Treatment and Ubiquitination Assay



E3 Ubiquitin Ligase AIP4 Mediates Ubiquitination of CXCR4


References


Author Index


Subject Index