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DNA Technology The Awesome Skill

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ISBN-10: 0120489201

ISBN-13: 9780120489206

Edition: 2nd 2001 (Revised)

Authors: I. Edward Alcamo

List price: $99.95
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Description:

DNA technology has reached into myriad fields, including genetic diagnoses, medical forensics and gene therapy. This work surveys the uses of gene technology in these fields.
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Book details

List price: $99.95
Edition: 2nd
Copyright year: 2001
Publisher: Elsevier Science & Technology Books
Publication date: 4/26/2000
Binding: Hardcover
Pages: 348
Size: 8.50" wide x 10.98" long x 0.35" tall
Weight: 2.376
Language: English

Preface
The Roots of DNA Research
Introduction
Inheritance Factors
Chromosomes and Factors
Genes and DNA
Relating DNA to Heredity
Bacterial Transformations
The Hershey-Chase Experiment
Summary
The Double Helix
Introduction
The Structure of DNA
The Components of DNA
DNA in Three Dimensions
DNA Replication
Summary
DNA in Action
Introduction
Foundation Studies
The One Gene-One Enzyme Hypothesis
Proteins and Codes
The Intermediary
The Genetic Code
Protein Synthesis
Transcription
Translation
Gene Control
Gene Complexity
Summary
Foundations of DNA Technology
Introduction
The Tools of Genetic Engineering
The Organisms
Microbial Recombinations
Restriction Enzymes
Ligases
Plasmids
Recombinant DNA Experiments
The Safety Issue
The Future
Summary
Methods of DNA Technology
Introduction
The Biochemistry of Gene Expression
Obtaining the Gene
Selecting the Vector
Selecting the Host Cell
Expressing the Gene
Collecting the Gene Product
Gene Libraries
Establishing a Library
Screening the Gene Library
The cDNA Library
Summary
Pharmaceutical Products of DNA Technology
Introduction
Human Protein Replacements
Insulin
Human Growth Hormone
Factor VIII
Human Therapies
Tissue Plasminogen Activator (TPA)
Interferon
Antisense Molecules
Other Innovative Pharmaceuticals
Vaccines
Hepatitis B
Acquired Immune Deficiency Syndrome
Other Traditional Vaccines
DNA Vacines
Summary
DNA Analysis and Diagnosis
Introduction
Methods of DNA Analysis
DNA Probes
The Polymerase Chain Reaction
Signal Amplification
The DNA Chip
RFLP Analysis
Diagnosing Infectious Disease
Acquired Immune Deficiency Syndrome
Tuberculosis
Lyme Disease
Human Papilloma and Other Diseases
Identifying Genetic Disease
Cystic Fibrosis
Duchenne's Muscular Dystrophy
Huntington's Disease
Fragile X Syndrome
Triple Repeat Disease
Retinoblastoma
Alzheimer's Disease
Amyotrophic Lateral Sclerosis
Diabetes
Cancer
Obesity
Hearing and Vision Loss
Other Human Diseases
Gene Banking
Environmental Monitoring
Summary
Gene Therapy
Introduction
Vectors in Gene Therapy
Advances in Gene Therapy
The ADA Experiments
Cancer Therapy with Genes
Therapy for Cystic Fibrosis
Therapy for AIDS
Other Endeavors
Safety Assurance
The Future
Summary
Medical Forensics and DNA Sleuthing
Introduction
DNA Matching Techniques
DNA Fingerprinting
Genetic Identifications
Microbial Identifications
DNA Windows to the Past
Ancient and Specimen DNA
Mitochondrial and Human Origins
Disputing the Neanderthal Connection
The First Americans
Other Studies of Anthropology
Abe Lincoln's DNA
Miscellaneous DNA Searches
Summary
The Agricultural Revolution
Introduction
DNA Insertion Methods
The Nitrogen Connection
Enhancing Resistance in Plants
The Ice-Minus Experiments
Resistance to Biological Agents
Resistance to Herbicides
Bioengineered Foods
The Flavr Savr Tomato
Other Innovative Foods
Vegetable Vaccines
"Pharm Animals"
Summary
Transgenic Animals
Introduction
Placing DNA in Mammalian Cells
Custom-Made Animals
The Human Mouse
The Oncomouse
The Alzheimer's Mouse
The Knockout Mouse
Animal Bioreactors
Human Hemoglobin from Pigs
Other Transgenic Animal Products
Other Bioreactors
"Dolly" and the Transgenic Clones
Building a Better Animal
Increasing Animal Resistance
Environmental Replacements
Summary
The Human Genome Project
Introduction
Deciphering the Human Genome
Origin of the Project
Whose Genome?
Whose Money?
Gene Patents
Mapping the Human Genome
The Gene Linkage Map
The Physical Map
Sequencing the Genome
Completed Genomes
The Study of Genomes
The Future of Genome Research
Summary
Glossary
Credits
Index