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| Preface | |
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| Laboratory Safety | |
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| Laboratory Safety Agreement | |
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| Basic Microbiology | |
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| Introduction to Microbiology | |
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| Ubiquity of Microorganisms | |
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| Pure Culture and Aseptic Technique | |
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| Introduction to the Compound Light Microscope | |
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| The Oil Immersion Lens | |
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| Introduction to Staining of Microorganisms | |
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| Simple Stains: Positive and Negative-Stains | |
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| Differential and Other Special Stains | |
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| Introduction to Microbial Growth | |
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| Chemically Defined, Complex, Selective, and Differential Media | |
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| Quantification of Microorganisms | |
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| Introduction to the Environment and Microbial Growth | |
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| Aerobic and Anaerobic Growth | |
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| The Effect of Incubation Temperature on Generation Time | |
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| Introduction to Control of Microbial Growth | |
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| Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time | |
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| Control of Microbial Growth with Ultraviolet Light | |
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| Changes in Osmotic Pressure Due to Salt and Sugar and Their Effects on Microbial Growth | |
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| Antibiotics | |
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| Antiseptics and Disinfectants | |
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| Introduction to Microbial Genetics | |
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| Selection of Bacterial Mutants Resistant to Antibiotics | |
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| Transformation: A Form of Genetic Recombination | |
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| Gene Regulation: Induction and Catabolite Repression | |
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| The Other Microbial World | |
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| Introduction to the Other Microbial World | |
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| Microscopic Identification of Fungi | |
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| Parasitology: Protozoa and Helmindis | |
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| Titering Prokaryotic Viruses | |
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| Microbes and Humans | |
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| Introduction to Medical Microbiology | |
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| Normal Skin Biota | |
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| Streptococci and Respiratory Microorganisms | |
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| Identification of Enteric Gram-Negative Rods | |
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| Clinical Unknown Identification | |
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| Immunology | |
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| Introduction to Some Immunological Principles and Techniques | |
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| Differential White Blood Cell Stains | |
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| Lysozyme, an Enzyme Contributing to Innate Immunity | |
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| Enzyme-Linked Immunosorbent Assay (ELISA) | |
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| Public Health | |
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| Introduction to the Prevention and Control of Communicable Diseases | |
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| Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques | |
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| Epidemiology | |
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| Applications of Biotechnology | |
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| Introduction to Biotechnology | |
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| Identifying DNA with Restriction Enzymes and the Polymerase Chain Reaction (PCR) | |
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| Electrophoresis | |
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| Identification of Bacteria Using the Ribosomal Database Project | |
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| Microarray | |
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| Individual Projects | |
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| Introduction to the Individual Projects | |
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| Hydrocarbon-Degrading Bacteria: Cleaning Up after Oil Spills | |
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| Luminescent Bacteria: Bacteria That Produce Light | |
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| Appendices | |
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| Culture Media and Reagent Formulas | |
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| Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in This Manual | |
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| Dilution Practice Problems | |
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| Alternative Procedures | |
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| Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size | |
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| History and Working Principles of Light Microscopy | |
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| Index | |