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Laboratory Exercises in Microbiology

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ISBN-10: 0072556803

ISBN-13: 9780072556803

Edition: 6th 2005 (Revised)

Authors: John P. Harley

List price: $90.31
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Book details

List price: $90.31
Edition: 6th
Copyright year: 2005
Publisher: McGraw-Hill Higher Education
Publication date: 2/24/2004
Binding: Comb Bound 
Pages: 480
Size: 8.75" wide x 10.50" long x 0.75" tall
Weight: 2.134
Language: English

John Harley teaches Anatomy & Physiology and General Biology at Eastern Kentucky University, Richmond, KY and is also co-author of Prescott/Harley/Klein Microbiology and the ASM Microbial Telecourse Study Guide.

Microscopic Techniques
Bright-Field Light Microscope and Microscopic Measurement of Organisms
The Hanging Drop Slide and Bacterial Motility
Dark-Field Light Microscope
Phase-Contrast Light Microscope
Flourescence Microscope
Bacterial Morphology and Staining
Negative Staining
Smear Preparation and Simple Staining
Gram Stain
Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures
Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)
Capsule Staining
Flagella Staining: West and Difco's SpotTest Methods
Basic Laboratory and Culture Techniques
Microbiological Culture Media Preparation and Sterilization
Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures
Spread-Plate Technique
The Streak-Plate Technique and Different Media
Pour-Plate Technique
Cultivation of Anaerobic Bacteria
Determination of Bacterial Numbers
Biochemical Activities of Bacteria
Carbohydrates I: Fermentation and B-Galactosidase Activity
Carbohydrates II: Triple Sugar Iron Agar Tests
Carbohydrates III: Starch Hydrolysis
Lipids: Lipid Hydrolysis
Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility
Proteins, Amino Acids, and Enzymes II: The IMViC Tests
Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis
Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis
Proteins, Amino Acids, and Enzymes V: Catalase Activity
Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity
Proteins, Amino Acids, and Enzymes VII: Oxidase Test
Proteins, Amino Acids, and Enzymes VIII: Urease Activity
Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Test
Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination
Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction
Rapid Multitest Systems
The API 20E System
The Enterotube II System
Unknown Identification
Using the First Edition of Bergey's Manual of Systematic Bacteriology to Identify Bacteria
General Unknown
Environmental Factors Affecting Growth of Microorganisms
Temperature
pH
Osmotic Pressure
The Effects of Chemical Agents on Bacteria I: Disinfectants
The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method)
Hand Washing, Environmental Sampling, and Microbiological Monitoring
Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods
Environmental and Food Microbiology
Standard Coliform Most Probably Number (MPN)Test and Presence-Absence Coliform Test
Membrane Filter Technique for Coliform and Fecal Streptococci;KONFIRM Test for Fecal Coliforms
Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers
Enumeration of Soil Microorganisms
Bacterial Count of a Food Product
Examination of Milk for Bacteria
Medical Microbiology
Agglutination Reactions: Blood Groups
Isolation of Normal Microbiota from the Human Body
Staphylococci
Pneumococci
Streptococci
Neisseriae
Aerobic and Anaerobic Endospore-Forming Bacteria
Survey of Selected Eucaryotic Microorganisms
Fungi I: Yeasts
Fungi II: Phycomycetes, Ascomycetes, and Basidiomycetes
Microbial Genetics and Genomics
Bacterial Mutation
Bacterial Transformation
Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids
Isolation of Genomic DNA from Saccharomyces cerevisiae
Isolation and Purification of Genomic DNA from Escherichia coli
Identifying Archaea and Bacteria Using the Internet and Computer-Assisted Gene Analysis
Dilutions with Sample Problems
Metric an
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