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Experiments in Biochemistry A Hands-on Approach

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ISBN-10: 0030212847

ISBN-13: 9780030212840

Edition: 2000

Authors: Shawn O. Farrell, Ryan T. Ranallo

List price: $102.95
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In EXPERIMENTS IN BIOCHEMISTRY: A HANDS-ON APPROACH you will find a myriad or resources that will prepare you for the challenges biochemistry offers! This biochemistry textbook offers a comprehensive set of experiements, practice problems, tip boxes and "Essential Information" boxes.
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Book details

List price: $102.95
Copyright year: 2000
Publisher: Brooks/Cole
Publication date: 4/6/1999
Binding: Hardcover
Pages: 344
Size: 8.50" wide x 11.00" long x 0.71" tall
Weight: 2.068
Language: English

Mary K. Campbell is Professor Emeritus of chemistry at Mount Holyoke College, where she taught biochemistry, general chemistry, and physical chemistry, as well as advised undergraduates working on biochemical research projects. Her avid interest in writing led to the publication of many highly successful editions of this textbook. Originally from Philadelphia, Dr. Campbell received her Ph.D. from Indiana University and completed postdoctoral work in biophysical chemistry at Johns Hopkins University. Her areas of interest include researching the physical chemistry of biomolecules, specifically, spectroscopic studies of protein-nucleic acid interactions. She is also coauthor with Shawn Farrell on BIOCHEMISTRY, 7e (Cengage Learning).Shawn O. Farrell, a native of Northern California, received his B.S. in biochemistry from University of California, Davis, studying carbohydrate metabolism. He completed his Ph.D. in biochemistry at Michigan State University, where he focused on the study of fatty acid metabolism. Dr. Farrell became interested in biochemistry while in college, as it was relevant to his passion for bicycle racing. He raced competitively for 15 years and now officiates bicycle races worldwide. He has taught biochemistry lecture and laboratory courses at Colorado State University for 16 years and now works for USCycling. Professor Farrell has written scientific journal articles about specific research projects and about laboratory teaching, as well as articles for sports publications, such as "Salmon, Trout, and Steelheader" magazine. He is co-author with Mary Campbell on BIOCHEMISTRY, 7e (Cengage Learning).

Introduction to the Text. Objectives of the Biochemistry Laboratory. 1. Biochemistry Boot Camp. Lab Safety. Scientific Notation. Significant Figures. Statistics and Scientific Measurements. Units. Concentration of Solutions. Dilutions. Graphing. Pipets and Pipetmen. Experiment #1: Use of Pipettors. 2. Acids, Bases, and Buffers. Strong Acids and Bases. Weak Acids and Bases. Polyprotic Acids. Buffers. Good Buffers. Choosing a Buffer. Effect of Concentration and Temperature. How We Make Buffers. The Big Summary. Why Is This Important?. Expanding the Topic. Experiment #2: Preparation of Buffers. 3. Spectrophotometry. Absorption of light. Beer-Lambert Law. Standard Curves. Protein Assays. Why Is This Important?. Expanding the Topic--Calculating concentrations from graphs. Tricks of the Trade: Choosing Test Tubes and Pipets. Experiment #3: Beer's Law and Standard Curves. Experiment #3B: Protein Concentration of LDH Fractions. 4. Enzyme Purification. Enzymes as Catalysts. Enzyme Purification. Units of Enzyme Activity. Calculating Initial Velocity. Purification Tables. Assay for Lactate Dehydrogenase (LDH). Why is this important?. Tricks of the Trade. Experiment #4: Purification of LDH (short version). Experiment #4A: Purification of LDH (comprehensive version). 5. Ion Exchange Chromatography. Amino Acids as Weak Acids and Bases. Isoelectric Points. Ion Exchange Chromatography. Ion Exchange Resins. Identification of Compounds Eluted from Columns. Thin Layer Chromatography. Why Is This Important?. Experiment #5: Separation and Identification of Amino Acids. Experiment #5A: Purification of LDH with Ion Exchange Chromatography. 6. Affinity Chromatography. Affinity Chromatography. Gel Supports. Affinity Ligands. Elution of bound molecules. Why Is This Important?. Experiment #6: Affinity Chromatography of LDH. 7. Gel Filtration Chromatography. Gel Filtration. Types of Supports. Determining the Molecular Weight. Distribution Coefficients. Why Is This Important?. Expanding the Topic. Experiment #7: Gel Filtration Chromatography. Experiment #7A: Gel Filtration Chromatography of LDH. 8. Enzyme Kinetics. Reaction Tates. Order of Reactions. Michaelis-Menten Approach. Significance of Km and Vmax. Linear Plots. Properties of Tyrosinase. Why Is This Important?. Experiment #8: Enzyme Kinetics of Tyrosinase. Experiment #8A: Enzyme Kinetics of LDH. 9. Electrophoresis. Electrophoresis. Agarose Gels. Polyacrylamide Gels. SDS. Staining Gels. Lactate Dehydrogenase. Why Is This Important?. Expanding the topic. Experiment #9: Native Gel Separation of LDH Isozymes (short version). Experiment #9A: Native Gel Separation of LDH Isozymes (comprehensive). Experiment #9B: SDS-Polyacrylamide Gel Electrophoresis (short version). Experiment #9C: SDS-Polyacrylamide Gel Electrophoresis (comprehensive). 10. Western Blots. 10/1 Western Blot Theory. 10/2 Antibodies/10/3 Color development. 10/4 Blocking and Washing. 10/5 Why Is This Important?. Experiment #10: Western Blot of Serum Proteins. Experiment #10a ; Western Blot of LDH. 11. Restriction Enzymes. Restriction nucleases. Restriction maps. Agarose Gel Separation of DNA. Staining DNA. Phage DNA. Why Is This Important?. Experiment #11: Analysis of DNA Restriction Fragments. 12. Cloning and Expression of Foreign Proteins. Recombinant DNA. Vectors. Foreign DNA. Restriction Enzymes. Cell Lines. Transformation. Selection. Expression. Why Is This Important?. Experiment #12: Cloning and Expression of Barracuda LDH-A. 13. Polymerase Chain Reaction. Amplification of DNA. Taq Polymerase. Primers. Changing Restriction Sites. Why Is This Important?. Experiment #13: PCR of Barracuda LDH-A.