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miRNAs Targeting and Targeting miRNAs | |
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miRNA Biology | |
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miRNAs Biogenesis | |
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miRNAs Actions | |
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miRNA Expression, Mutation and Polymorphism | |
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miRNA Expression | |
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miRNA Mutation | |
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miRNA Polymorphism | |
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miRNAs and Human Disease | |
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miRNAs and Developmental Disorders | |
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miRNAs and Apoptosis | |
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miRNAs and Cancer | |
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miRNAs and Cardiovascular Disease | |
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miRNAs and Neuronal Disease | |
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miRNAs and Viral Disease | |
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miRNAs and Metabolic Disorders | |
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miRNA and Epigenetics | |
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miRNAs as Therapeutic Targets | |
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Strategies for Therapeutic Modulation of miRNAs | |
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Approaches for Therapeutic Modulation of miRNAs | |
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References | |
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miRNA Interference Technologies: An Overview | |
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New Concepts of miRNAi Technologies | |
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�miRNAi�, A New Concept | |
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�miRNA as a Regulator of a Cellular Function�, Second New Concept | |
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�One-Drug, Multiple-Target�, Third New Concept | |
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�miRNA Seed Family�, Another New Concept | |
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General Introduction to miRNAi Technologies | |
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miRNA-Targeting Technologies | |
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Targeting-miRNA Technologies | |
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miRNAi Technologies in Basic Research and Drug Design | |
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References | |
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Synthetic Canonical miRNA Technology | |
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Introduction | |
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Protocols | |
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Designing SC-miRNAs | |
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Validating SC-miRNAs | |
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Principle of Actions | |
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Applications | |
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Advantages and Limitations | |
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References | |
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miRNA Mimic Technology | |
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Introduction | |
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Protocols | |
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Designing miR-Mimics | |
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Validating miR-Mimics | |
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Principle of Actions | |
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Applications | |
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Advantages and Problems | |
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References | |
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Multi-miRNA Hairpins and Multi-miRNA Mimics Technologies | |
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Introduction | |
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Protocols | |
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Construction of Multi-miRNA Hairpins | |
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Construction of Multi-miRNA Mimics (Chen et al. 2009) | |
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Principle of Actions | |
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Applications | |
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Advantages and Limitations | |
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References | |
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miRNA Transgene Technology | |
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Introduction | |
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Protocols | |
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Conventional Transgene Methods | |
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Artificial Intronic miRNA Methods | |
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Cre-loxP Knock-in Methods | |
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Principle of Actions | |
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Conventional Transgene Methods | |
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Artificial Intronic miRNA Methods | |
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Applications | |
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Advantages and Limitations | |
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References | |
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Anti-miRNA Antisense Oligonucleotides Technology | |
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Introduction | |
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Protocols | |
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Designing AMOs | |
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Modifying AMOs | |
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Monitoring Delivery Efficiency of AMOs | |
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Evaluating Functional Effectiveness of AMOs | |
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Principle of Actions | |
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Applications | |
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Advantages and Limitations | |
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References | |
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Multiple-Target Anti-miRNA Antisense Oligonucleotides Technology | |
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Introduction | |
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Protocols | |
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Designing MT-AMOs | |
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Validating MT-AMOs | |
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Principle of Actions | |
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Applications | |
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Advantages and Limitations | |
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References | |
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miRNA Sponge Technology | |
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Introduction | |
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Protocols | |
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Designing miRNA Sponges | |
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Validating miRNA Sponges | |
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Principle of Actions | |
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Applications | |
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Advantages and Problems | |
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References | |
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miRNA-Masking Antisense Oligonucleotides Technology | |
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Introduction | |
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Protocols | |
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Principle of Actions | |
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Applications | |
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Advantages and Limitations | |
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References | |
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Sponge miR-Mas Technology | |
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Introduction | |
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Protocols | |
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Principle of Actions | |
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Applications | |
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Advantages and Limitations | |
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Reference | |
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miRNA Knockout Technology | |
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Introduction | |
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Protocols | |
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Homologous Recombination Methods | |
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Cre-loxP Methods | |
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FLP-FRT Deletion Methods | |
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Principle of Actions | |
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Applications | |
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Advantages and Limitations | |
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References | |
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Dicer Inactivation Technology | |
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Introduction | |
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Protocols | |
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Neomycin-Expression Cassette Methods (Homologous Recombination) | |
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Cre-loxP Methods | |
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Principle of Actions | |
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Applications | |
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Advantages and Limitations | |
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References | |
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Index | |