Molecular Diagnostics For the Clinical Laboratorian

ISBN-10: 1588293564
ISBN-13: 9781588293565
Edition: 2nd 2005
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Description: This updated and expanded tutorial guide to molecular diagnostic techniques takes advantage of many new molecular technologies to include both improved traditional methods and totally new methods, some not yet in routine use. The authors offer  More...

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Book details

Edition: 2nd
Copyright year: 2005
Publisher: Humana Press
Publication date: 4/16/2012
Binding: Hardcover
Pages: 567
Size: 8.75" wide x 11.50" long x 1.25" tall
Weight: 4.158
Language: English

This updated and expanded tutorial guide to molecular diagnostic techniques takes advantage of many new molecular technologies to include both improved traditional methods and totally new methods, some not yet in routine use. The authors offer cutting-edge molecular diagnostics for genetic disease, human cancers, infectious diseases, and identity testing, as well as new insights into the question of quality assurance in the molecular diagnostics laboratory. Additional chapters address other technologies found in the clinical laboratory that complementary to molecular diagnostic technologies and discuss genetic counseling and the ethical and social issues involved with nucleic acid testing. A value-added compact disk containing a companion e-book version of the book, is included for downloading and use in the reader's PC or PDA.

Dedication
Foreword
Preface
Color Plates
Contributors
Value Added eBook Instructions
Introduction
An Historical Perspective on the Clinical Diagnostic Laboratory
Basic Molecular Biology
An Overview of Nucleic Acid Chemistry, Structure, and Function: The Foundations of Molecular Biology
Extraction of Nucleic Acids
Nucleic Acid Blotting Techniques: Theory and Practice
The Polymerase Chain Reaction
Bioinformatics: Computer-Based Approaches to Genetic Analysis
Molecular Diagnostic Technologies
PCR-Based Methods for Mutation Detection
Alternative Methods for Amplified Nucleic Acid Testing
Electrophoretic Methods for Mutation Detection and DNA Sequencing
Single-Nucleotide Polymorphisms: Testing DNA Variation for Disease Association
Microarray Approaches to Gene Expression Analysis
Methods for Analysis of DNA Methylation
Other Clinical Diagnostic Technologies
Flow Cytometry
Medical Cytogenetics
Fluorescence In Situ Hybridization: A Major Milestone in Luminous Cytogenetics
Immunohistochemistry
Laser Capture Microdissection
Quality Assurance in the Molecular Diagnostics Laboratory
Framework for Quality Assurance in Molecular Diagnostics
Verification of Molecular Assays
Standards and Standardization of Molecular Diagnostics
Laboratory-Developed Tests in Molecular Diagnostics
Applications of Molecular Diagnostics for Genetic Diseases
An Overview of Molecular Genetics
Genetic Basis of Neurologic and Neuromuscular Diseases
Molecular Mechanisms of Endocrine Disorders
Molecular Pathogenesis of Cardiovascular Disease
Molecular Diagnostics in Coagulation
Cystic Fibrosis
Prenatal Genotyping for Identification of Fetuses at Risk for Immune Cytopenic Disorders
Personalized Medicine
Applications of Molecular Diagnostics for Human Cancers
Molecular Pathogenesis of Human Cancer
Application of Molecular Diagnostics to Hereditary Nonpolyposis Colorectal Cancer
Molecular Genetic Applications to the Diagnosis of Lymphoma
Molecular Genetic Abnormalities in Acute and Chronic Leukemias
Applications of Molecular Diagnostics for Infectious Diseases
Molecular Testing for Chlamydia trachomatis and Neisseria gonorrhoeae
Human Papillomavirus
Molecular Diagnosis for HIV-1
Hepatitis C
Cytomegalovirus
Applications of Molecular Diagnostics for Identity-Based Testing
HLA Typing Using Molecular Methods
Molecular Analysis for Forensic Casework and Parentage Testing
Molecular Assessment of Bone Marrow Transplant Engraftment
Use of DNA-Based Identity Testing for Specimen Identification
Issues for the Clinical Molecular Pathology Laboratory
Genetic Counseling Considerations in Molecular Diagnosis
Ethical, Social, and Legal Issues Related to Molecular Genetic Testing
Index

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