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Preface | |
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Frequently Used Markers | |
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Introduction | |
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The Basics of Doing a Cross | |
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Fly Chromosomes | |
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Recognizing Markers | |
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Nomenclature | |
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Evolution of Gene Names | |
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What Makes Flies So Great? Balancer Chromosomes | |
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Deciphering Mating Schemes | |
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Basic Fly Husbandry | |
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Coddling Difficult Strains | |
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Collecting Flies for Crosses | |
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Isolating New Variants | |
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Principles | |
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Mutagens | |
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Ethylmethanesulfonate | |
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Ethylnitrosourea | |
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Radiation | |
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Insertional Mutagenesis | |
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Genetic Schemes for Detecting Mutants | |
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New Alleles at an Autosomal Locus | |
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New Alleles on the X | |
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Identifying New Genes by Mutagenesis | |
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Isogenizing the Starter Stock | |
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The Mutagenesis Screen | |
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Screening the X for New Genes | |
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Insertional Mutagenesis and Enhancer Trapping | |
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Enhancer-trap Screens | |
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New Insertional Alleles of Known Loci | |
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Mutating Genes for Which There Are No Allelic Differences | |
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Screening Over a Deletion of the Locus | |
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Targeted Insertional Mutagenesis | |
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Homologous Recombination | |
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RNA Interference | |
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Searching for Gain of Function | |
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Generating Mutants: One Size Does Not Fit All | |
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Mapping | |
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Segregation Analysis | |
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Problems with Balancers | |
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Meiotic Mapping | |
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Deletion Mapping | |
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Duplication Mapping | |
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Synthesizing Deletions and Duplications | |
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Identifying New Embryonic Genes by Deletion Mapping | |
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Mapping P-Element Insertion Sites | |
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Fine-Grain Traditional Meiotic Mapping with P-Element Markers | |
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Mapping by P-Element-Induced Male Recombination | |
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Fine-Grain Mapping by Transposon-Induced Deletions | |
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Mapping by DNA Polymorphisms | |
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Synthesizing Specific Genotypes | |
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Principles | |
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Simple Manipulations of a Single Chromosome | |
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Linking Mutations | |
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Manipulating Two Chromosomes | |
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Pitfalls | |
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How Many Flies? | |
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Which Sex? | |
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Manipulating Three Chromosomes | |
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Making Rearrangements | |
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Inducing Deletions | |
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Synthesizing Stable Deletions from T(Y;A)s | |
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Synthesizing Deletions from P-element Insertions | |
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Inducing Duplications | |
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Synthesizing Stable Duplications from T(Y;A)s | |
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Synthesizing Attached-Xs | |
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Autosynaptic Chromosomes and the Joys of Gibberish | |
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Analysis of Mutations: I. Characteristics of the Allele | |
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Formal Classification of Mutations | |
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Principles | |
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Null Alleles (Amorphs) | |
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Hypomorphs | |
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Hypermorphs | |
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Neomorphs | |
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Antimorphs | |
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Gain Versus Loss of Function | |
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Intragenic Interactions | |
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Interacting Loci | |
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Ordering Genes in a Pathway | |
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Dosage-dependent Interactions | |
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Conditional Alleles | |
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Inducible Promoters | |
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Maintenance of Mutant Phenotypes | |
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Genetic Background | |
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Analysis of Mutations: II. Mosaics | |
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Principles | |
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Chromosome Loss | |
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Uses of Chromosome Loss Mosaics | |
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Fate Mapping | |
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Focus of Gene Action | |
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Methods for Inducing Chromosome Loss | |
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Ring Loss | |
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Chromosome Loss Mosaics for Autosomal Genes | |
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paternal-loss (pal) | |
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claret-non-disjunctional (cand) | |
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Care of Mosaic-producing Stocks | |
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Mitotic Recombination | |
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Synchronizing Age of Cultures | |
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Uses of Mitotic Recombination Mosaics | |
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Lineage Analysis | |
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Focus of Gene Action | |
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Time of Gene Action | |
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Methods for Producing Mitotic Recombinant Clones | |
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Markers for Internal Tissues | |
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GAL4 and Expression Mosaicism | |
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Cell Ablations | |
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GAL4 Targeted FLP/FRT Recombination | |
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Isolating Tissue-targeted Mutations Using Mitotic Recombination | |
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P-element Replacement for Exploitation of Existing Enhancer Traps | |
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Commencement | |
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Solutions to Problems | |
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Glossary | |
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Fly Resources | |
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References | |
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Index | |