Fly Pushing The Theory and Practice of Drosophila Genetics

ISBN-10: 0879697113

ISBN-13: 9780879697112

Edition: 2nd 2004

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Book details

List price: $61.00
Edition: 2nd
Copyright year: 2004
Publisher: Cold Spring Harbor Laboratory Press
Publication date: 7/7/2004
Binding: Comb Bound 
Pages: 192
Size: 6.50" wide x 9.00" long x 0.75" tall
Weight: 1.298
Language: English

Jonathan Flint is a Professor at the Wellcome Trust Centre for Human Genetics, Oxford University. He has extensive experience in molecular biology, molecular genetics, and rodent behavioural genetics with a specific focus on models of anxiety. Ralph Greenspan is based at the NeuroscienceInstitute in San Diego. He has studied the genetic foundations of many behaviours in Drosophila, and has analyzed naturally occurring genetic variations in its behaviour. Kenneth Kendler is a Professor at the Virginia Institute for Psychiatric and Behavioral Genetics at the Virginia CommonwealthUniversity. He has conducted family and twin studies on a range of psychiatric and drug use disorders, and has conducted gene finding studies for schizophrenia, alcoholism, and drug dependence.

Preface
Frequently Used Markers
Introduction
The Basics of Doing a Cross
Fly Chromosomes
Recognizing Markers
Nomenclature
Evolution of Gene Names
What Makes Flies So Great? Balancer Chromosomes
Deciphering Mating Schemes
Basic Fly Husbandry
Coddling Difficult Strains
Collecting Flies for Crosses
Isolating New Variants
Principles
Mutagens
Ethylmethanesulfonate
Ethylnitrosourea
Radiation
Insertional Mutagenesis
Genetic Schemes for Detecting Mutants
New Alleles at an Autosomal Locus
New Alleles on the X
Identifying New Genes by Mutagenesis
Isogenizing the Starter Stock
The Mutagenesis Screen
Screening the X for New Genes
Insertional Mutagenesis and Enhancer Trapping
Enhancer-trap Screens
New Insertional Alleles of Known Loci
Mutating Genes for Which There Are No Allelic Differences
Screening Over a Deletion of the Locus
Targeted Insertional Mutagenesis
Homologous Recombination
RNA Interference
Searching for Gain of Function
Generating Mutants: One Size Does Not Fit All
Mapping
Segregation Analysis
Problems with Balancers
Meiotic Mapping
Deletion Mapping
Duplication Mapping
Synthesizing Deletions and Duplications
Identifying New Embryonic Genes by Deletion Mapping
Mapping P-Element Insertion Sites
Fine-Grain Traditional Meiotic Mapping with P-Element Markers
Mapping by P-Element-Induced Male Recombination
Fine-Grain Mapping by Transposon-Induced Deletions
Mapping by DNA Polymorphisms
Synthesizing Specific Genotypes
Principles
Simple Manipulations of a Single Chromosome
Linking Mutations
Manipulating Two Chromosomes
Pitfalls
How Many Flies?
Which Sex?
Manipulating Three Chromosomes
Making Rearrangements
Inducing Deletions
Synthesizing Stable Deletions from T(Y;A)s
Synthesizing Deletions from P-element Insertions
Inducing Duplications
Synthesizing Stable Duplications from T(Y;A)s
Synthesizing Attached-Xs
Autosynaptic Chromosomes and the Joys of Gibberish
Analysis of Mutations: I. Characteristics of the Allele
Formal Classification of Mutations
Principles
Null Alleles (Amorphs)
Hypomorphs
Hypermorphs
Neomorphs
Antimorphs
Gain Versus Loss of Function
Intragenic Interactions
Interacting Loci
Ordering Genes in a Pathway
Dosage-dependent Interactions
Conditional Alleles
Inducible Promoters
Maintenance of Mutant Phenotypes
Genetic Background
Analysis of Mutations: II. Mosaics
Principles
Chromosome Loss
Uses of Chromosome Loss Mosaics
Fate Mapping
Focus of Gene Action
Methods for Inducing Chromosome Loss
Ring Loss
Chromosome Loss Mosaics for Autosomal Genes
paternal-loss (pal)
claret-non-disjunctional (cand)
Care of Mosaic-producing Stocks
Mitotic Recombination
Synchronizing Age of Cultures
Uses of Mitotic Recombination Mosaics
Lineage Analysis
Focus of Gene Action
Time of Gene Action
Methods for Producing Mitotic Recombinant Clones
Markers for Internal Tissues
GAL4 and Expression Mosaicism
Cell Ablations
GAL4 Targeted FLP/FRT Recombination
Isolating Tissue-targeted Mutations Using Mitotic Recombination
P-element Replacement for Exploitation of Existing Enhancer Traps
Commencement
Solutions to Problems
Glossary
Fly Resources
References
Index
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