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| Dedication | |
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| Preface | |
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| Acknowledgments | |
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| How We Learned That DNA Is the Genetic Material | |
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| How We Learned the Function of DNA | |
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| How We Learned How Genes Are Regulated | |
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| Basic Tools and Techniques of DNA Science | |
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| Methods for Finding and Expressing Important Genes | |
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| Modern Methods for Analyzing Whole Genomes | |
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| The DNA Science of Cancer | |
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| Applying DNA Science to Human Genetics and Evolution | |
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| Laboratories: Lab Safety and Adherence to National Institutes of Health Guidelines | |
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| Measurements, Micropipetting, and Sterile Techniques | |
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| Bacterial Culture Techniques | |
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| Isolation of Individuals Colonies | |
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| Overnight Suspension Culture | |
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| Mid-log Suspension Culture | |
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| DNA Restriction Analysis | |
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| Effects of DNA Methylation on Restriction | |
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| Rapid Colony Transformation of E. coli with Plasmid DNA | |
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| Assay for an Antibiotic Resistance Enzyme | |
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| Purification and Identification of Recombinant GFP | |
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| Purification of GFP by HIC | |
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| PAGE Analysis of Purified GFP | |
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| Purification and Identification of Plasmid DNA | |
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| Plasmid Minipreparation of pAMP | |
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| Restriction Analysis of Purified pAMP | |
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| Recombination of Antibiotic Resistance Genes | |
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| Restriction Digest of Plasmids pAMP and pKAN | |
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| Ligation of pAMP and pKAN Restriction Fragments | |
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| Transformation of E. coli with Recombinant DNA | |
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| Classic Procedure for Preparing Competent Cells | |
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| Transformation of E. coli with Recombinant DNA | |
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| Replica Plating to Identify Mixed E. coli Populations | |
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| Purification and Identification of Recombinant DNA | |
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| Plasmid Minipreparation of pAMP/pKAN Recombinants | |
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| Restriction Analysis of Purified Recombinant DNA | |
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| Answers to Discussion Questions | |
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| Appendices | |
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| Equipment, Supplies, and Reagents | |
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| Recipes for Media, Reagents, and Stock Solutions | |
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| Restriction Map Data for pAMP, pKAN, pBLU, pGREEN, and Bacteriophage [lambda] | |
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| Cautions | |
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| Name Index | |
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| Subject Index | |