DNA Science A First Course

ISBN-10: 0879696362
ISBN-13: 9780879696368
Edition: 2nd 2003
List price: $45.00
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Book details

List price: $45.00
Edition: 2nd
Copyright year: 2003
Publisher: Cold Spring Harbor Laboratory Press
Binding: Hardcover
Pages: 575
Size: 9.50" wide x 11.50" long x 1.50" tall
Weight: 4.092
Language: English

Dedication
Preface
Acknowledgments
How We Learned That DNA Is the Genetic Material
How We Learned the Function of DNA
How We Learned How Genes Are Regulated
Basic Tools and Techniques of DNA Science
Methods for Finding and Expressing Important Genes
Modern Methods for Analyzing Whole Genomes
The DNA Science of Cancer
Applying DNA Science to Human Genetics and Evolution
Laboratories: Lab Safety and Adherence to National Institutes of Health Guidelines
Measurements, Micropipetting, and Sterile Techniques
Bacterial Culture Techniques
Isolation of Individuals Colonies
Overnight Suspension Culture
Mid-log Suspension Culture
DNA Restriction Analysis
Effects of DNA Methylation on Restriction
Rapid Colony Transformation of E. coli with Plasmid DNA
Assay for an Antibiotic Resistance Enzyme
Purification and Identification of Recombinant GFP
Purification of GFP by HIC
PAGE Analysis of Purified GFP
Purification and Identification of Plasmid DNA
Plasmid Minipreparation of pAMP
Restriction Analysis of Purified pAMP
Recombination of Antibiotic Resistance Genes
Restriction Digest of Plasmids pAMP and pKAN
Ligation of pAMP and pKAN Restriction Fragments
Transformation of E. coli with Recombinant DNA
Classic Procedure for Preparing Competent Cells
Transformation of E. coli with Recombinant DNA
Replica Plating to Identify Mixed E. coli Populations
Purification and Identification of Recombinant DNA
Plasmid Minipreparation of pAMP/pKAN Recombinants
Restriction Analysis of Purified Recombinant DNA
Answers to Discussion Questions
Appendices
Equipment, Supplies, and Reagents
Recipes for Media, Reagents, and Stock Solutions
Restriction Map Data for pAMP, pKAN, pBLU, pGREEN, and Bacteriophage [lambda]
Cautions
Name Index
Subject Index

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