Introduction to Forensic DNA Analysis

ISBN-10: 0849302331
ISBN-13: 9780849302336
Edition: 2nd 2002 (Revised)
List price: $119.95 Buy it from $19.86
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Description: This new edition of a bestseller emphasizes the advantages and limitations of various DNA techniques used in the analysis of forensic evidence. It covers the shift to Short Tandem Repeat (STR) technology, and updates methods and legal issues  More...

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Book details

List price: $119.95
Edition: 2nd
Copyright year: 2002
Publisher: CRC Press LLC
Publication date: 12/21/2001
Binding: Hardcover
Pages: 312
Size: 7.25" wide x 10.25" long x 1.00" tall
Weight: 1.870
Language: English

This new edition of a bestseller emphasizes the advantages and limitations of various DNA techniques used in the analysis of forensic evidence. It covers the shift to Short Tandem Repeat (STR) technology, and updates methods and legal issues affected by this shift. The authors discuss forensic DNA issues from both a scientific and legal perspective, and present the material in a manner understandable by professionals in the legal system, forensic science, and law enforcement.

The Nature of Physical Evidence
Science and the Law
Principles and Processes of Criminalistics
The Principles
The Processes
Identification
Classification and Individualization
Association
Reconstruction
Fingerprints and DNA
Conventional Blood Typing
The Collection and Preservation of Physical Evidence
Extraneous Substances
Collection of Evidence
Preservation of Evidence
Evaluation of Evidence
RFLP
PCR
A Short History of DNA Typing
The Scientific Basis of DNA Typing
Why DNA?
An Introduction to Human Genetics
The Physical Basis of Heredity
Alleles: Variations on a Theme
Population Genetics
An Introduction to the Molecular Biology of DNA
Two Kinds of Variation
Enzymes: The Workhorses of the Biological World
An Overview of Forensic DNA Typing Systems
RELP Analysis
PCR Amplification
HLA DQ[alpha]/HLA DQA1
AmpliType PM
D1S80
STRs
Gender Identification
Y-STRs
Mitochondrial DNA
What Kinds of Samples Can be Analyzed?
How Much Sample Do You Need?
Procedures for Forensic DNA Analysis
Isolation of DNA
Chelex Extraction
QiaAmp Extraction
Organic Extraction
Differential Extraction
Determining the Quality and Quantity of DNA
Determination of Quantity
Determination of Quality
RFLP Analysis
PCR Amplification
Analysis of PCR Product
Sequence Polymorphisms
AmpliType PM+DQA1
Mitochondrial DNA (mtDNA)
Length Polymorphisms (D1S80, STRs, Gender ID)
Automated Analysis Systems
Automated DNA Extraction and Amplification
Liquid Blood Transfer
DNA Extraction
Quantitation
Amplification
Robot Issues
Summary of Automated Procedures
Interpretation of DNA Typing Results
Complicating Factors
Multiple Contributors
Sexual Assault Evidence
Non-sexual Assault Mixtures
Degradation
Extraneous Substances
Chemical Inhibition of Enzymes
Non-human DNA
System-Specific Interpretational Issues
RFLP
Multi-(More Than Two) Banded Patterns
Single-Banded Patterns
Summary of RFLP Interpretational Issues
PCR Systems
Nuclear DNA
Mitochondrial DNA
Summary of PCR Systems Interpretational Issues
Summary of DNA Interpretation Issues
Assessing the Strength of the Evidence
Determination of Genetic Concordance
Continuous Allele Systems
Discrete Allele Systems
Evaluation of Results
Frequency Estimate Calculations
Population Substructure
Estimating Frequencies
Continuous Allele Systems (RFLP)
Discrete Allele Systems (all PCR systems)
Correction Factors
Relatives
Counting Method
Error Rates
Likelihood Ratios
What Is the Question?
Complex Evidence
Mixtures
Articulating the Hypothesis
When Is a DNA Profile Unique
The DNA Databank
Premise of a Databank
The Difference Between a Databank and a Database
Elements of a Successful Databank
Legislation
Collection of Samples
Offenders
Cases
Analysis of Samples
Choice of Markers
Offenders
Cases
Transforming Analyzed Data into a Database
The Computer Program
Communication Between Laboratories
Privacy
Flow of Offender Data into the Databank
Import of Profiles into the Database
Categories and Indexes
Searching Profiles
Confirmation of a Match
United States Statistics
Summary
Quality Assurance
Certification and Accreditation
Certification
Accreditation
SWDAM (Formerly TWGDAM)
NRC I and II
NRC I
NRC II
Federal DNA Advisory Board
Admissibility Standards: Science on Trial in the Courtroom
Frye, Daubert, and the Federal Rules of Evidence
The Frye Standard
The Federal Rules of Evidence
The Daubert Standard
Past Admissibility
DNA: Some Landmark Cases
RFLP
PCR: DQ[alpha]/A1, Polymarker, D1S80
PCR: STRs
Mitochondrial DNA
The State of the Debate
Epilogue
Glossary
Key Phrases
Assessment Tools
Chromosomal Locations
Profile Frequency Calculation
Likelihood Ratio and Probability of Exclusion
DAB 98
DAB Statistics
NRC I, II
DNA Statutes
DNA Decision Summary
List STR decisions
Internet Resources
Index

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