Laboratory DNA Science

ISBN-10: 0805330402
ISBN-13: 9780805330403
Edition: 1st 1996
List price: $125.33
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Book details

List price: $125.33
Edition: 1st
Copyright year: 1996
Publisher: Benjamin-Cummings Publishing Company
Publication date: 11/7/1995
Binding: Paperback
Pages: 371
Size: 8.50" wide x 11.00" long x 0.75" tall
Weight: 2.244
Language: English

Basic Techniques. Labs
Measurements, Micropipetting, and Sterile Techniques
Basic Culture Techniques
DNA Restriction and Electrophoresis
Dna Modification
Effects of DNA Methylation on Restriction
Plasmid Transformation and Identification
Rapid Colony Transformation of E. coli with Plasmid DNA
Purification and Identification of Plasmid DNA
Plasmid Recombination and Identification
Recombination of Antibiotic Resistance Genes
Transformation of E. coli with Recombinant DNA
Replicating to Identify Mixed E. coli Populations
Purification and Identification of Recombinant Plasmid DNA
Restriction Mapping of Linear and Circular Chromosomes
Restriction Mapping of the l Chromosome
Restriction Mapping of the Plasmid pBR322
Southern Hybridization
Southern Hybridization of l DNA
Genomic Library Construction and Analysis
Construction of Genomic Library of l DNA
Transformation of E. coli with l Library
Colony Hybridization of l Library
Purification and Identification of l Clones
Cloning by Polymerase Chain Reaction
Amplification and Purification of l DNA Fragment
Recombination of PCR Product and Plasmid pBLU
Transformation of E. coli with PCR/pBLU Product
The Purification and Identification of PCR/pBLU Recombinants
Human Dna Fingerprinting
Detection of an Alu Insertion Polymorphism by Polymerase Chain Reaction
Detection of a VNTR Polymorphism by Polymerase Chain Reaction
Equipment, Supplies, and Reagents
Recipes for Media, Reagents, and Stock Solutions
Restriction Map Data for pAMP, pKAN, pBLU, l and Bacteriophage
Sequence Data for pAMP, pKAN, pBLU, and Bacteriophage l

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