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| Foreword | |
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| Preface | |
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| Beginnings: Simplicity and Elegance, DNA and Protein | |
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| Proteins | |
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| DNA | |
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| Simple Organisms | |
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| Further Reading | |
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| The Code for Life: DNA to Protein | |
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| Genes Code for Proteins | |
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| Defining the Coding Problem: The Information People | |
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| DNA to RNA to Protein: The Biochemical Pathway People | |
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| Cracking the Code: Synthetic mRNA | |
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| The Genetic Code: God Has an Orderly Mind | |
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| Further Reading | |
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| Turning Genes on and Off: Genes That Control Other Genes | |
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| Bacterial Growth and the Induced Synthesis of [beta]--galactosidase: The Monod Group | |
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| The Problem of Lysogeny and Phage [lambda]: The Jacob Group | |
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| Bacterial Sex and Erotic Induction | |
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| Turning Off Related Genes: The Road to the Repressor and the Operon | |
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| The Discovery of Messenger RNA | |
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| What Is the Repressor? | |
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| Some Comments on the Road to the Operon | |
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| Positive and Negative Regulation: Two Ways to Run an Operon | |
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| Multi-Operon Regulation: The Glucose Effect | |
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| Regulatory Diversity: Review and Preview | |
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| Further Reading | |
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| Replicating the Genome | |
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| DNA Polymerase Replicates DNA | |
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| The Problems of Replicating a Genome | |
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| The Genetics of Replication | |
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| The Fine Structure of DNA Replication | |
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| The "Real" Replication Enzyme Is Just Like Pol I | |
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| A Simple Task That Needs Many Proteins: Priming and Elongating | |
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| Special Proteins Needed for Genome Replication: Stretching, Unwinding, Untwisting | |
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| Replicating Duplex DNA | |
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| Starting Genome Replication: A Special Structure | |
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| Further Reading | |
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| Making RNA from DNA | |
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| The Flow of Biological Information | |
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| The Transcription Enzyme: RNA Polymerase | |
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| The Properties of mRNA | |
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| Developmental Pathways of Phage | |
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| Lambdology: The Developmental Biology of a Field | |
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| Lambdology: The Developmental Biology of a Phage | |
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| Lambdology as a Community | |
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| The Lac and [lambda] Repressors Bind to Operator DNA | |
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| The Biochemistry of RNA Polymerase | |
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| Positive and Negative Regulators of the lac Operon | |
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| The General View of Regulatory Proteins: 1972 | |
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| The Idea of Antitermination Regulation: Problems in Paradise | |
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| Regulated Termination in the trp Operon: The Attenuator | |
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| Transcription Termination and How to Avoid It: [lambda] N and Q Proteins | |
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| The Regulator-Operator Interaction: How to Locate a Site on DNA | |
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| A New Approach to DNA Sequence and Its Recognition: Single-Cleavage Analysis | |
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| Determining DNA Sequence by DNA Replication | |
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| The Molecular Study of Protein-DNA Interactions | |
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| Building a Regulatory Protein: A DNA-Binding Motif | |
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| The Regulator-Polymerase Interaction: General Rules? | |
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| Further Reading | |
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| The RNA World: New Proteins and Revised RNAs | |
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| The Anticodon: Base-Pairs Can Wobble | |
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| Base Sequence of tRNA: A Three-Leaf Clover for Holley | |
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| Message Decoding: Methods and Protagonists | |
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| The Problem of Starting a Protein | |
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| Ending a Protein: The Value of Nonsense | |
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| Protein Translation Factors: Turning the Gears in Protein Synthesis | |
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| Using the Ribosome: GTP and Translocation | |
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| The Ribosome: The Many Faces of an Enigma | |
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| RNA Slicing in Bacteria | |
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| RNA Splicing in Eukaryotes | |
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| The Biochemistry of Splicing: Lariats on a Spliceosome | |
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| RNA Processing Without Proteins: Catalytic RNA | |
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| Further Reading | |
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| DNA on Its Own: Genetic Recombination | |
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| General Recombination and the Holliday Model | |
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| Holliday Intermediates in Cells | |
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| Genes and Enzymes in Bacterial Recombination | |
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| The RecA Protein and Branch Migration | |
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| The RecBCD Protein: Unwinding and Nicking | |
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| General Recombinational Pathways: The Future | |
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| Site-Specific Recombination and the Campbell Model | |
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| Prophage Excision and Excisive Recombination | |
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| The Biochemistry of Site-Specific Recombination | |
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| DNA-Protein Interactions in Site-Specific Recombination | |
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| Other Site-Specific Recombinases | |
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| Transposition and Replicative Site-Specific Recombination | |
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| The IS Insertion Elements | |
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| Mu: A Phage That Does Not Integrate Like Lambda | |
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| Identifying the Mu Components Needed for Transposition | |
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| A Unifying Model for Transposition | |
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| Transposition: Why Have It? | |
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| Further Reading | |
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| Regulating the Regulators: Developmental and Salvational Decisions | |
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| The Lysis-Lysogeny Decision by Phage [lambda]: cII as the Master Regulator | |
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| Regulating the Regulator: Control of cII Activity | |
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| Molecular Decision Making: Primary and Secondary Switches | |
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| Lifestyle Crises and Salvational Decisions | |
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| The Heat Shock Response and Molecular Chaperones | |
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| Heat Shock: From Cell Biology to Molecular Biology | |
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| Regulation and Function of Heat Shock Proteins in E. coli | |
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| Regulation of the Heat Shock Response: New Promoter Recognition by RNA Polymerase | |
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| Regulation of the Regulator: Post-Transcriptional Control of [sigma superscript 32] | |
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| The Heat Shock Response in Eukaryotic Cells | |
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| Regulated DNA Repair and Mutagenesis | |
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| The UVR Pathway of Excision Repair | |
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| RecA-Mediated Recombinational Repair | |
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| The SOS Response to DNA Damage | |
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| The Biochemistry of SOS Regulation: Induction by Repressor Cleavage | |
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| Induced Mutation in the SOS Response | |
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| Further Reading | |
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| Making DNA from RNA: The Strange Life of the Retrovirus | |
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| Quantitative Animal Virology: The Plaque and Focus Assays | |
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| Making DNA from RNA | |
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| The Path from Virus to Provirus and Back Again | |
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| The Wild Ride of Reverse Transcriptase | |
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| The Retrovirus and Its Cancer Gene | |
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| Retroviruses as Transposons | |
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| The HIV Retrovirus | |
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| Further Reading | |
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| Genetic Engineering: Genes and Proteins on Demand | |
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| The Social Context for Basic Research in Biology | |
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| A Biological Revolution from the Unpredictable | |
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| Hitchhiking Genes and Multiplying Vectors | |
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| Biological Roots: Phage with Bacterial Genes and Cohesive Sites | |
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| Biological Roots: Restriction Enzymes for Pieces of DNA | |
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| The Engineered Joining of DNA Molecules | |
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| DNA Cloning: Amplification of Foreign DNA by a Plasmid Vector | |
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| Safety Concerns and Regulatory Policy | |
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| Genetic Engineering: A Giant Leap for Eukaryotic Molecular Biology | |
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| The Industrial and Medical Ramifications of Genetic Engineering | |
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| Further Reading | |
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| Afterword | |
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| Timeline | |
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| Glossary | |
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| Notes | |
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| Name Index | |
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| Subject Index | |