Flow Cytometry First Principles

ISBN-10: 0471382248
ISBN-13: 9780471382249
Edition: 2nd 2001 (Revised)
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Description: This second edition reflects the recent advances in flow cytometry and includes a new chapter on the staining of intracellular proteins, including cytokines. The new laser options and new fluorochromes are also discussed.

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Book details

Edition: 2nd
Copyright year: 2001
Publisher: John Wiley & Sons, Incorporated
Publication date: 7/23/2001
Binding: Paperback
Pages: 296
Size: 6.25" wide x 9.50" long x 0.50" tall
Weight: 1.166
Language: English

This second edition reflects the recent advances in flow cytometry and includes a new chapter on the staining of intracellular proteins, including cytokines. The new laser options and new fluorochromes are also discussed.

Preface
Acknowledgments (First Edition)
Acknowledgments (Second Edition)
The Past as Prologue
Setting the Scene
Instrumentation: Into the Black Box
Illumination of the Stream
Centering Cells in the Illuminating Beam
Detection of Signals from Cells
Electronics
Information: Harnessing the Data
Data Storage
Data Analysis
Seeing the Light: Lasers, Fluorochromes, and Filters
General Theory
Lasers
Fluorochromes
Partitioning the Signal with Lenses, Filters, and Mirrors
Spectral Compensation
Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating
Cells from Blood
Staining for Surface Markers
Controls
Quantitation
Sensitivity
The Strategy of Gating
Gating on Fluorescence
Cells from Within: Intracellular Proteins
Methods for Permeabilizing Cells
Examples of Intracellular Staining
Cells from Within: DNA in Life and Death
Fluorochromes for DNA Analysis
Ploidy
Cell Cycle Analysis
Two-Color Analysis for DNA and Another Parameter
Chromosomes
Apoptosis
Necrosis
The Sorting of Cells
Sorting Theory
Characterization of Sorted Cells
Alternative Methods for Sorting
The Condition of Cells After Sorting
Disease and Diagnosis: The Clinical Laboratory
The Hematology Laboratory
The Pathology Laboratory
Solid Organ Transplantation
Comments
Out of the Mainstream: Research Frontiers
Functional Assays
The Aquatic Environment
Reporter Molecules
Microbiology
Molecular Biology
Multiplex Cytometry for Soluble Analytes
Reproductive Technology
Flowing On: The Future
General References
Glossary
Figure Credits
Index

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