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Culture of Animal Cells A Manual of Basic Technique

ISBN-10: 0471348899
ISBN-13: 9780471348894
Edition: 4th 2000
Authors: R. Ian Freshney
List price: $105.00
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Description: This interactive laboratory based reference will complement the printed volume by utilizing selected portions of the book, accompanied by an extensive photomicrographic atlas of cells and video.

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Book details

List price: $105.00
Edition: 4th
Copyright year: 2000
Publisher: John Wiley & Sons, Incorporated
Publication date: 3/20/2000
Binding: Hardcover
Pages: 624
Size: 7.50" wide x 11.50" long x 1.50" tall
Weight: 3.784
Language: English

This interactive laboratory based reference will complement the printed volume by utilizing selected portions of the book, accompanied by an extensive photomicrographic atlas of cells and video.

Figures
Color Plates
Preface
Abbreviations
Introduction
Background
Advantages of Tissue Culture
Limitations
Major Differences In Vitro
Types of Tissue Culture
Biology of Cultured Cells
The Culture Environment
Cell Adhesion
Cell Proliferation
Differentiation
Energy Metabolism
Initiation of the Culture
Evolution of Cell Lines
The Development of Continuous Cell Lines
Origin of Cultured Cells
Design and Layout
Planning
Construction and Services
Layout
Equipment
Requirements of a Tissue Culture Laboratory Essential Equipment
Beneficial Equipment
Useful Additional Equipment
Consumable Items
Aseptic Technique
Objectives of Aseptic Technique
Elements of Aseptic Environment
Sterile Handling
Laminar Flow
Standard Procedure
Apparatus and Equipment
Safety
Risk Assessment
General Safety
Fire
Radiation
Biohazards
Culture Vessels
The Substrate
Choice of Culture Vessel
Specialized Systems
Treated Surfaces
Media
Development of Media
Physicochemical Properties
Balanced Salt Solutions
Complete Media
Serum
Selection of Medium and Serum
Other Supplements
Serum-Free Media
Disadvantages of Serum
Advantages of Serum-Free Media
Disadvantages of Serum-Free Media
Replacement of Serum
Selection of Serum-Free Medium
Development of Serum-Free Medium
Preparation of Serum-Free Medium
Conclusions
Preparation and Sterilization
Apparatus
Reagents and Media
Control, Testing, and Storage of Media
Primary Culture
Types of Primary Cell Culture
Isolation of the Tissue
Primary Culture
Cell Lines
Nomenclature
Subculture and Propagation
Immortalization of Cell Lines
Cell Line Designations
Selection of Cell Line
Routine Maintenance
Cloning and Selection
Cloning
Stimulation of Plating Efficiency
Suspension Cloning
Isolation of Clones
Replica Plating
Selective Inhibitors
Isolation of Genetic Variants
Interaction with Susbstrate
Cell Separation
Cell Density and Isopyknic Sedimentation
Antibody-Based Techniques
Magnetic Sorting
Cell Size and Sedimentation Velocity
Centrifugal Elutriation
Fluorescence-Activated Cell Sorting
Other Techniques
Beginner's Approach to Cell Separation
Characterization
The Need for Characterization
Morphology
Chromosome Analysis
DNA Content
RNA and Protein
Enzyme Activity
Antigenic Markers
Differentiation
Authentication
Differentiation
Expression of In Vivo Phenotype
Stages of Commitment and Differentiation
Proliferation and Differentiation
Commitment and Lineage
Markers of Differentiation
Induction of Differentiation
Differentiation and Malignancy
Practical Aspects
Transformation
Role in Cell Line Characterization
What is Transformation?
Genetic Instability
Immortalization
Aberrant Growth Control
Tumorigenicity
Contamination
Sources of Contamination
Types of Microbial Contamination
Monitoring for Contamination
Eradication of Contamination
Cross-Contamination
Conclusions
Cryopreservation
Need for Cryopreservation
Preservation
Cell Banks
Transporting Cells
Quantitation
Cell Counting
Cell Weight
DNA Content
Protein
Rates of Synthesis
Preparation of Samples for Enzyme Assay and Immunoassay
Cytometry
Replicate Sampling
Cell Proliferation
Plating Efficiency
Labeling Index
Cell Cycle Time (Generation Time)
Cell Migration
Cytotoxicity
Introduction
In Vitro Limitations
Nature of the Assay
Anticancer Drug Screening
Transformation
Inflammation
Specialized Cells
Epithelial Cells
Mensenchymal Cells
Neuroectodermal Cells
Hematopoietic Cells
Gonads
Tumor Cells
Sampling
Disaggregation
Primary Culture
Characterization
Development of Cell Lines
Selective Culture
Specific Tumor Types
Organotypic Culture
Cell Interaction and Phenotypic Expression
Organ Culture
Histotypic Culture
Filter-Well Inserts
Cultures of Neuronal Aggregates
Organotypic Culture
Scale-Up
Scale-up in Suspension
Scale-up in Monolayer
Specialized Techniques
Lymphocyte Preparation
Autoradiography
Time-Lapse Recording
Confocal Microscopy
Cell Synchrony
Culture of Amniocytes
Culture of Cells from Poikilotherms
Molecular Techniques
Molecular Biology in Cell Culture
In Situ Molecular Hybridization
Somatic Cell Fusion
Production of Monoclonal Antibodies
DNA Transfer
Problem Solving
Slow Cell Growth
Choice of Medium
Unstable Reagents
Purity of Constituents
Plastics
Glassware
Microbial Contamination
Chemical Contamination
Primary Culture
Cloning
Differentiation
Feeding
Subculture
Cross-Contamination
Cryopreservation
Granularity of Cells
Cell Counting
Viability
In Conclusion
Reagent Appendix
Trade Index
Glossary
References
Index

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