Principles and Practice of Biological Mass Spectrometry

ISBN-10: 0471330531
ISBN-13: 9780471330530
Edition: 2001
Authors: Chhabil Dass
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Description: This volume provides a treatment of the principles and applications of mass spectrometry to bio-related fields. It contains a description of the latest mass spectrometry-based techniques.

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Book details

List price: $223.95
Copyright year: 2001
Publisher: John Wiley & Sons, Incorporated
Publication date: 10/30/2000
Binding: Hardcover
Pages: 448
Size: 6.25" wide x 9.25" long x 1.25" tall
Weight: 1.694
Language: English

This volume provides a treatment of the principles and applications of mass spectrometry to bio-related fields. It contains a description of the latest mass spectrometry-based techniques.

Foreword
Series Preface
Preface
Abbreviations
Introduction to Mass Spectrometry
Historical Perspective
Why Mass Spectrometry?
Basic Concepts of Mass Spectrometry
The Nature of Mass Spectrometry Data
Applications
Literature
Ionization Methods
Electron Ionization
Basic Principles of Electron Ionization
Limitations of Electron Ionization
Chemical Ionization
Charge-Exchange Chemical Ionization (CECI)
Mixed Chemical Ionization
Negative-Ion Chemical Ionization
Limitations of Chemical Ionization
Desorption Ionization
Field Desorption
Plasma Desorption Ionization
Fast-Atom Bombardment
Matrix-Assisted Laser Desorption Ionization
Spray Techniques
Thermospray Ionization
Atmospheric Pressure Chemical Ionization
Electrospray Ionization
Comparison of Different Ionization Methods
Mass Analysis and Ion Detection
Magnetic Sector Mass Spectrometers
Kinetic Energy of Ions
Magnetic Analyzer
Electrostatic Analyzer
Double-Focusing Magnetic Sector Mass Spectrometers
Quadrupole Mass Spectrometers
Principle of Operation
Mass Range
Mass Resolution
RF-Only quadrupole
Quadrupole Ion-Trap Mass Spectrometer
Principle of Operation
Extension of Mass Range and Resolution of an Ion Trap
Coupling with ESI and MALDI Techniques
Time-of-Flight Mass Spectrometer
Principle of Operation
Mass Resolution
Reflectron
Orthogonal Acceleration TOF Mass Spectrometer
Time-Delay Ion Extraction for Structure Elucidation Applications
Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
Principle of Operation
High-Mass Analysis Capabilities
High-Mass Resolution Capability
Ion Mobility Mass Spectrometry
A Comparison of Important Characteristics of Mass Analyzers
Ion Detection
Faraday Cup Detector
Secondary Electron Multipliers
Postacceleration Detector
Low-Temperature Calorimetric Detectors for High-Mass Ions
Focal Plane Detectors
Tandem Mass Spectrometry
Basic Concepts
Types of Scans
Instrumentation for Tandem Mass Spectrometry
Magnetic Sector Tandem Mass Spectrometers
Triple-Sector Quadrupole
Hybrid Tandem Mass Spectrometers
Tandem Mass Spectrometry with Time-of-Flight Instrument
Tandem Ion-Trap Mass Spectrometry
Modes of Ion Activation
Metastable Ions
Collision-Induced Dissociation
Other Modes of Ion Activation
Molecular Mass Measurement
Definition of Molecular Mass
Monoisotopic versus Average Mass
Resolving Power
High-Resolution Mass Spectrometers
Exact Mass Measurements
High-Resolution Exact Mass Measurements by Magnetic Sector Instruments
High-Resolution Exact Mass Measurements by MALDI-TOFMS
High-Resolution Exact Mass Measurements by FT-ICRMS
Exact Mass Measurements at Low Resolution with a Quadrupole Mass Filter
Mass Measurement of Biopolymers
Mass Measurements of Megadalton-Sized Molecules
Mass Calibration Standards
Quantitative Analysis
Calibration
Internal Standard
Mass Spectrometry Measurements
Selected-Ion Monitoring
Selected-Reaction Monitoring
Liquid Chromatography/Mass Spectrometry for Quantitative Analysis
Nanoelectrospray Ionization for Quantitative Analysis
High-Throughput LCMS and LCMSMS Analyses
Validation Studies
Coupling of Separation Techniques with Mass Spectrometry
General Considerations
Types of Mass Analyzers
The Types of Mass Spectrometry Signal
Mass Spectral Data Acquisition
Gas Chromatography/Mass spectrometry
Basic Principles of Gas Chromatography
Coupling of Gas Chromatography with Mass Spectrometry
Applications
High-Performance Liquid Chromatography/Mass Spectrometry
Basic Principle of HPLC Separation
Coupling of Liquid Chromatography with Mass Spectrometry
Coupling of Liquid Chromatography with Electrospray Ionization Mass Spectrometry
Atmospheric Pressure Chemical Ionization Interface
Capillary Electrophoresis/Mass Spectrometry
Basic Principle of Capillary Electrophoresis
Coupling of Capillary Electrophoresis with Mass Spectrometry
Affinity Chromatography Combined with Mass Spectrometry
Other Separation Techniques Combined with Mass Spectrometry
Structural Analysis of Proteins
Biological Functions and Structures of Proteins
Traditional Biochemical Approaches
A General Protocol for Determination of the Primary Structure of Proteins
Purification of Proteins
Molecular Mass Measurement of Proteins
Peptide Mapping
Reduction and Carboxymethylation
Cleavage of Proteins
Fractionation of Protein Digest by RP-HPLC
Analysis of Peptide Maps
Protein Identification by Gel Electrophoresis--Mass Spectrometry
Mass Spectrometry Characterization of Gel-Separated Proteins
Protein Identification by Database Searching
De Novo Protein Sequencing
Proteomics
Automated High-throughput Analysis of Proteins
Characterization of Mutations in Proteins
Disulfide Bond Location in Proteins
Determination of the Number of Disulfide Bonds
Generation of Disulfide-Containing Peptides
Identification of Disulfide-Containing Peptides
Sequence Verification of Disulfide-Containing Peptides
Analysis of Phosphoproteins
Traditional Approaches for the Analysis of Phosphoproteins
Mass Spectometry Protocal for Analysis of Phosphoproteins
Structural Characterization of Peptides
Traditional Approaches for Analysis of Peptides
Older Mass Spectrometry Methods
Fragmentation Characteristics of Peptides
Characterization of Peptides by FABMS
Sequence Determination by FAB and Conventional Mass Spectrometry
Chemical Derivatization of Peptides for FAB Analysis
Characterization of Peptides by ESIMS
Characterization of Peptides by MALDIMS
Sequencing of Peptides by MALDIMS
Sequence Determination by Tandem Mass Spectrometry
Sequence Information from a Mass Spectrum
Study of Metal Ion Adducts
De Novo Peptide Sequencing
Electron Capture Dissociation
Peptide Ladder Sequencing
Generation of Peptide Ladders
Analysis of Glycoproteins and Oligosaccharides
Structural Features of Glycans and Glycoproteins
Analysis of Glycoproteins
Determination of the Molecular Mass of Glycoproteins
Identification of the Presence, Type, and Sites of Glycosylation
Release of Carbohydrate Side Chains from Glycopeptides
FABMS Analysis
ESIMS Analysis
MALDIMS Analysis
Tandem Mass Spectrometry Analysis
Structural Characterization of the Carbohydrate Side Chains
Linkage Analysis by GCMS
FABMS Analysis
Analysis by ESIMS and MALDIMS
Characterization by Molecular Mass and Database Search
Sequential Digestion Methods
Collision-Induced Dissociation for Structural Analysis of Carbohydrates
Postsource Decay for Structural Analysis of Carbohydrates
Folding-Unfolding and Higher-order Structures of Proteins
Conventional Methods for Study of Conformational Changes in Proteins
Charge-State Distribution as a Measure of Conformation of Proteins
Reactivity of Some Functional Groups as a Measure of Conformation of Proteins
Hydrogen Exchange as a Probe of Conformational Changes in Proteins
Amide Hydrogen Exchange Rate as a Probe of the Protein Structures
Conformational Dynamics of Proteins
Mass Spectrometry Measurements of Amide Hydrogen Exchange Rates
Time-Resolved Electrospray Ionization Mass Spectrometry for Study of Conformational Changes
Matrix-Assisted Laser Desorption/Ionization for Study of Conformational Changes
Peptide Mass Mapping for Study of Conformational Changes
Ion Mobility Measurements for Study of Conformational Changes
Investigation of Noncovalent Interactions
Conventional Methods for Study of Noncovalent Complexes
Mass Spectrometry-Based Approaches for Study of Noncovalent Interactions
Electrospray Ionization for Study of Noncovalent Complexes
Matrix-Assisted Laser Desorption/Ionization for Study of Noncovalent Complexes
Hydrogen/Deuterium Isotope Exchange for Study of Noncovalent Complexes
Proteolytic Cleavage for Study of Noncovalent Complexes
Determination of Binding Affinities
Absolute Values of Binding Constants
Protein Epitope Mapping
Applications
Protein-Protein Complexes
Protein-Peptide and Peptide-Peptide Complexes
Protein-Small-Molecule Complexes
Polypeptide-Metal Ion Complexes
Noncovalent Complexes of Oligonucleotides
Noncovalent Complexes of Proteins and Oligonucleotides
Structure Determination of Lipids and Glycolipids
A General View of Lipid Structures
Mass Spectrometry Analysis of Fatty Acids and Glycerides
Mass Spectrometry Analysis of Phospholipids
Fast-Atom Bombardment Mass Spectrometry Analysis of Phospholipids
Electrospray Ionization-Mass Spectrometry of Phospholipids
Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry of Phospholipids
Liquid Chromatography-Mass Spectrometry Analysis of Phospholipids
Mass Spectrometry of Glycolipids
Mass Spectrometry of Lipopolysaccharides
Mass Spectrometry Analysis of Steroids
Mass Spectrometry Analysis of Prostaglandins
Mass Spectrometry Analysis of Leukotrienes
Screening Combinatorial Libraries
Combinatorial Synthetic Procedures
General Considerations in Analysis of Combinatorial Libraries
Mass Spectrometry Analysis of Support-Bound Libraries
High-throughput Screening Protocols
Screening of Combinatorial Libraries
Characterization of Oligonucleotides
Traditional Methods of Oligonucleotide Sequencing
Older Mass Spectrometry Techniques
Degradation of Nucleic Acids and Oligonucleotides
Molecular Mass Determination of Oligonucleotides
Electrospray Ionization for Molecular Mass Determination
MALDI for Molecular Mass Determination
Comparison of ESIMS and MALDIMS for Analysis of Oligonucleotides
Base Composition from Mass Measurement
Sequencing of Nucleic Acids by Mass Spectrometry Techniques
Gas-Phase Sequencing Techniques
Sequencing Methods Based on Solution-Phase Reactions
Applications to Real-World Problems
Immunologic Studies
Direct Analysis of Bioactive Compounds from Biological Tissues
Direct Analysis of Peptides in Single Neurons
Direct Analysis of Peptides and Proteins from Biological Tissues
Direct Analysis of Single Mast Cells
Clinical Applications of Mass Spectrometry
Detection of Mutation of the Cystic Fibrosis Gene
Detection of Transthyretin Mutants
Characterization of Hemoglobin Variants
Detection of Enzyme-Deficiency Diseases
Mass Spectrometry in Detection of Cachexia
Rapid Screening of Protein Profiles of Human Breast Cancer Cell Lines
Analysis of Proteins in Human Cerebrospinal Fluid
Gender Identification of Human DNA Samples
Proteomic Analysis
Analysis of Microorganisms
Bacterial Analysis
Analysis of Viruses
Detection of Phosphorylated Peptides in Bovine Adrenal Medulla
Quantitative Analysis of Neuropeptides in Pituitary Tumors
Human Genome Project
Index

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