Current Protocols Essential Laboratory Techniques

ISBN-10: 047094241X

ISBN-13: 9780470942413

Edition: 2nd 2012

List price: $85.95
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Description:

The latest title from the acclaimed Current Protocols series, Current Protocols Essentail Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory.  From covering the basic skills such as measurement, preparation of reagents and use of basic insturmentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher.  Written by a combination of distinguished investigators and outstandning faculty, Current Protocols Essentail Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successfull research career.
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Book details

List price: $85.95
Edition: 2nd
Copyright year: 2012
Publisher: John Wiley & Sons, Limited
Publication date: 4/10/2012
Binding: Paperback
Pages: 664
Size: 8.50" wide x 10.75" long x 1.25" tall
Weight: 3.674
Language: English

Foreword
Preface
Contributors List
Conversion FactorsFlow Chart: Application of Techniques
Volume/Weight Measurement
Volume Measurement
Weight Measurement
Concentration Measurement
Spectrophotometry
Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Reagent Preparation
Theoretical Discussion
Practical Discussion
Recipes In-House
Cell Culture Techniques
Aseptic Technique
Culture of Escherichia coli and Related Bacteria
Sample Preparation
Centrifugation and Cell Fractionation In-House
Purification and Concentration of Nucleic Acids
Chromatography
Overview of Chromatography
Column Chromatography
Electrophoresis
Overview of Electrophoresis
Agarose Gel Electrophoresis
SDS-PAGE
Staining of Gels
Blotting
Overview of Blotting
Nucleic Acid Blotting: Southern and Northern
Protein Blotting: Immunoblotting
Labeling DNA and Probe Preparation
Microscopy
Conventional Light Microscopy
Immunofluorescence Microscopy
Enzymatic Reactions
Working with Enzymes
Overview of PCR
Real-Time PCR
Bioinformatics
Using NCBI Blast
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