Current Protocols Essential Laboratory Techniques

Name: Current Protocols Essential Laboratory Techniques
Availability: InStock
ISBN: 9780470942413

ISBN-10: 047094241X

ISBN-13: 9780470942413

Edition: 2nd 2012

Authors: Sean R. Gallagher, Emily A. Wiley

List price: $84.50

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Description:

The latest title from the acclaimed Current Protocols series, Current Protocols Essentail Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic insturmentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstandning…

Book details

List price: $84.50
Edition: 2nd
Copyright year: 2012
Publisher: John Wiley & Sons, Limited
Publication date: 4/10/2012
Binding: Paperback
Pages: 672
Size: 8.50" wide x 11.00" long x 1.30" tall
Weight: 3.344
Language: English



Foreword


Preface


Contributors List


Conversion FactorsFlow Chart: Application of Techniques




Volume/Weight Measurement



Volume Measurement




Weight Measurement




Concentration Measurement



Spectrophotometry




Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy




Reagent Preparation



Theoretical Discussion




Practical Discussion




Recipes In-House



Cell Culture Techniques



Aseptic Technique




Culture of Escherichia coli and Related Bacteria




Sample Preparation



Centrifugation and Cell Fractionation In-House



Purification and Concentration of Nucleic Acids




Chromatography



Overview of Chromatography




Column Chromatography




Electrophoresis



Overview of Electrophoresis




Agarose Gel Electrophoresis




SDS-PAGE




Staining of Gels




Blotting



Overview of Blotting




Nucleic Acid Blotting: Southern and Northern




Protein Blotting: Immunoblotting




Labeling DNA and Probe Preparation




Microscopy



Conventional Light Microscopy




Immunofluorescence Microscopy




Enzymatic Reactions



Working with Enzymes




Overview of PCR




Real-Time PCR




Bioinformatics



Using NCBI Blast