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| Preface to the fourth edition | |
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| Informational macromolecules | |
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| Information processing and molecular biology | |
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| Nucleic acid structure and function | |
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| Protein structure and function | |
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| Macromolecular assemblies | |
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| Analysis of proteins | |
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| Properties of nucleic acids | |
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| Chemical and physical properties of nucleic acids | |
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| Spectroscopic and thermal properties of nucleic acids | |
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| DNA supercoiling | |
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| Prokaryotic and eukaryotic chromosome structure | |
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| Prokaryotic chromosome structure | |
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| Chromatin structure | |
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| Eukaryotic chromosome structure | |
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| Genome complexity | |
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| DNA replication | |
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| DNA replication: an overview | |
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| Bacterial DNA replication | |
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| Eukaryotic DNA replication | |
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| DNA damage, repair, and recombination | |
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| DNA damage | |
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| Mutagenesis | |
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| DNA repair | |
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| Recombination and transposition | |
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| Transcription in bacteria | |
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| Basic principles of transcription | |
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| Escherichia coli RNA polymerase | |
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| The E. coli �<sup>70</sup> promoter | |
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| Transcription initiation, elongation, and termination | |
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| Regulation of transcription in bacteria | |
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| The lac operon | |
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| The trp operon | |
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| Transcriptional regulation by alternative a factors and RNA | |
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| Transcription in eukaryotes | |
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| The three RNA polymerases: characterization and function | |
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| RNA Pol I genes: the ribosomal repeat | |
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| RNA Pol III genes: 5S and tRNA transcription | |
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| RNA Pol II genes: promoters and enhancers | |
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| General transcription factors and RNA Pol II initiation | |
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| Regulation of transcription in eukaryotes | |
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| Eukaryotic transcription factors | |
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| Examples of transcriptional regulation | |
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| RNA processing and RNPs | |
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| rRNA processing and ribosomes | |
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| tRNA and other small RNA processing | |
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| mRNA processing, hnRNPs, and snRNPs | |
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| Alternative mRNA processing | |
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| The genetic code and tRNA | |
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| The genetic code | |
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| tRNA structure and function | |
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| Protein synthesis | |
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| Aspects of protein synthesis | |
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| Mechanism of protein synthesis | |
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| Initiation in eukaryotes | |
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| Translational control and post-translational events | |
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| Bacteriophages and eukaryotic viruses | |
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| Introduction to viruses | |
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| Bacteriophages | |
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| DNA viruses | |
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| RNA viruses | |
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| Cell cycle and cancer | |
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| The cell cycle | |
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| Oncogenes | |
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| Tumor suppressor genes | |
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| Apoptosis | |
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| Gene manipulation | |
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| DNA cloning: an overview | |
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| Preparation of plasmid DNA | |
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| Restriction enzymes and electrophoresis | |
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| Ligation, transformation, and analysis of recombinants | |
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| Cloning vectors | |
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| Design of plasmid vectors | |
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| Bacteriophages, cosmids, YACs, and BACs | |
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| Eukaryotic vectors | |
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| Gene libraries and screening | |
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| Genomic libraries | |
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| cDNA libraries | |
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| Screening procedures | |
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| Analysis and uses of cloned DNA | |
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| Characterization of clones | |
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| Nucleic acid sequencing | |
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| Polymerase chain reaction | |
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| Analysis of cloned genes | |
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| Mutagenesis of cloned genes | |
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| Functional genomics and the new technologies | |
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| Introduction to the 'omics | |
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| Global gene expression analysis | |
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| Proteomics | |
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| Cell and molecular imaging | |
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| Transgenics and stem cell technology | |
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| Bioinformatics | |
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| Systems and synthetic biology | |
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| Further reading | |
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| Abbreviations | |
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| Index | |