Protein Purification Principles and Practice

ISBN-10: 0387940723
ISBN-13: 9780387940724
Edition: 3rd 1994 (Revised)
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Description: The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using  More...

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Book details

List price: $144.00
Edition: 3rd
Copyright year: 1994
Publisher: Springer
Publication date: 11/19/1993
Binding: Hardcover
Pages: 380
Size: 6.50" wide x 9.75" long x 1.00" tall
Weight: 1.760
Language: English

The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand. Reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" -- Analytical Biochemistry "well organized and written clearly" -- American Scientist "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" -- Nature  

Prefaces
The Protein Purification Laboratory
Making an Extract
Analysis- Measurement of Protein and Enzyme Activity
Separation by Precipitation
Separation by Adsorption I: General Principles
Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents
Separation by Adsorption: Affinity Techniques
Separation in Solution
Purification of Special Types of Proteins
Small-Scale and Large-Scale Procedures
Analysis for Purity
Optimization of Procedures: Final Steps
Appendix A-D
References
Index

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