Biological Low-Voltage Scanning Electron Microscopy

Name: Biological Low-Voltage Scanning Electron Microscopy
Availability: InStock
ISBN: 9780387729701

ISBN-10: 0387729704

ISBN-13: 9780387729701

Edition: 2008

Authors: Heide Schatten, James B. Pawley

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Description:

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapter s in books. This comprehensive volume is…

Book details

List price: $169.99
Copyright year: 2008
Publisher: Springer New York
Publication date: 12/18/2007
Binding: Hardcover
Pages: 317
Size: 6.10" wide x 9.25" long x 0.35" tall
Weight: 1.496
Language: English



Preface


List of Contributors



The Early Development of the Scanning Electron Microscope




LVSEM for Biology




The Aberration-Corrected SEM




Noise and Its Effects on the Low-Voltage SEM




High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols




Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM




Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM




Cryo-SEM of Chemically Fixed Animal Cells




High-Resolution and Low-Voltage SEM of Plant Cells




High-Resolution Cryoscanning Electron Microscopy of Biological Samples




Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy



Index