Monoclonal Antibodies A Practical Approach

Name: Monoclonal Antibodies A Practical Approach
Availability: OutOfStock
ISBN: 9780199637232

ISBN-10: 0199637237

ISBN-13: 9780199637232

Edition: 2000

Authors: Philip S. Shepherd, Christopher Dean

List price: $220.00

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Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperienced immunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well as purification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in…

Book details

List price: $220.00
Copyright year: 2000
Publisher: Oxford University Press, Incorporated
Publication date: 7/13/2000
Binding: Hardcover
Pages: 510
Size: 7.60" wide x 9.60" long x 1.20" tall
Weight: 2.860
Language: English



List of protocols


Abbreviations



Preparation of Rodent Monoclonal Antibodies by in vitro Somatic Hybridization, Christopher Dean and Philip Shepherd



Preparation of Recombinant Antibodies From Immune Rodent Spleens and the Design of Their Humanisation by CDR Grafting



Appendix



Selection of Antibodies From Phage Libraries of Immunoglobulin Genes




Arm Complexes for in vitro Display and Evolution of Antibody Combining Sites




Human Monoclonal Antibodies to Blood Group Antigens




Laboratory Based Methods for Small-Scale Productions of Monoclonal Antibodies




Isolation and Purification of Antibodies From Tissue Culture Supernatant




Radiolabelling of Monoclonal Antibodies




Non-Radioactive Antibody Probes




Immunogold Probes for Light and Electron Microscopy




Characterization of Cellular Antigens Using Monoclonal Antibodies




Immunoassays




Immunoaffinity Chromatography of Macromolecules




Immunochemical Detection of BrdUrd-Labelled Nuclei for Monitoring Cell Kinetics




Immunofluorescence Microscopy




FACS Analysis of Clinical Haematological Samples in Transplantation for Cancer




Immunocytochemical Staining of Cells and Tissues for Diagnostic Applications




Detection of Chemically Modified DNA in Lymphocytes of Patients Undergoing Chemotheraphy




Monoclonal Antibody Therapy in Organ Transplantation




Monoclonal Antibody Therapy in Rheumatoid Arthritis



List of suppliers


Index