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Bioseparations Science and Engineering

ISBN-10: 0195123409
ISBN-13: 9780195123401
Edition: 2nd 2002
List price: $168.40 Buy it from $67.69
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Description: Designed for undergraduates, graduate students, and industry practitioners, Bioseparations Science and Engineering fills a critical need in the field. Current, comprehensive, and concise, it covers bioseparations unit operations in greater depth  More...

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Book details

List price: $168.40
Edition: 2nd
Copyright year: 2002
Publisher: Oxford University Press, Incorporated
Publication date: 10/31/2002
Binding: Hardcover
Pages: 432
Size: 8.75" wide x 9.75" long x 1.00" tall
Weight: 2.266
Language: English

Designed for undergraduates, graduate students, and industry practitioners, Bioseparations Science and Engineering fills a critical need in the field. Current, comprehensive, and concise, it covers bioseparations unit operations in greater depth than other texts on this topic. In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative description noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scaleup. Unique to this text is a chapter dedicated to bioseparations process design and economics, in which a process simular, SuperPro DesignerRG is used to analyze and evaluate the production of three important biological products. Other unique features include basic information about bioproducts and engineering analysis and a chapter with bioseparations laboratory exercises. Bioseparations Science and Engineering is ideal for students and professionals alike. Features BL Incorporates numerous example problems within the chapters BL Offers extensive sets of problems at the end of chapters BL Includes basic information about bioproducts BL Provides thorough coverage of analytical methods for bioproducts BL Uses the simulation software SuperPro DesignerRG to illustrate the analysis and evaluation of the production of citric acid, recombinant human insulin, and monoclonal antibodies BL Includes laboratory exercises that support text material BL Accompanied by a solutions manual available to instructors who adopt this text BL Supplemented by a website (www.biosep.ou.edu) with new problems and examples and links to useful databases and manufacturers of bioseparations equipment and supplies

Paul Todd has been teaching law for over 37 years, and is a Professor of Commercial and Maritime Law at the University of Southampton.

Preface
All chapters (except Chapter 12) end with the following sections: Summary, Nomenclature, Problems, and References
Preface
Introduction to Bioproducts and Bioseparations
Instructional Objectives
Broad Classification of Bioproducts
Small Biomolecules
Primary Metabolites
Secondary Metabolites
Summary of Small Biomolecules
Macromolecules: Proteins
Primary Structure
Secondary Structure
Tertiary Structure
Example
Effect of a Reducing Agent on Protein Structure and Mobility
Quaternary Structure
Prosthetic Groups and Hybrid Molecules
Functions and Commercial Uses of Proteins
Stability of Proteins
Recombinant Protein Expression
Macromolecules: Nucleic Acids and Oligonucleotides
Macromolecules: Polysaccharides
Particulate Products
Introduction to Bioseparations: Engineering Analysis
Stages of Downstream Processing
Example
:Initial Selection of Purification Steps
Basic Principles of Engineering Analysis
Process and Product Quality
Criteria for Process Development
The Route to Market
The Chemical and Applications Range of the Bioproduct
Documentation of Pharmaceutical Bioproducts
GLP and cGMP
Formulation
Analytical Methods
Instructional Objectives
Specifications
Assay Attributes
Precision
Accuracy
Specificity
Linearity, Limit of Detection, and Limit of Quantitation
Range
Robustness
Analysis of Biological Activity
Animal Model Assays
Cell-Line-Derived Bioassays
In Vitro Biochemical Assays
Example
:Coupled Enzyme Assay for Alcohol Oxidase
Analysis of Purity
Electrophoretic Analysis
Example
:Estimation of the Maximum Temperature in an Electrophoresis Gel
High Performance Liquid Chromatography (HPLC)
Mass Spectrometry
Coupling of HPLC with Mass Spectrometry
UV Absorbance
Example
:Determination of Molar Absorptivity
CHNO/Amino Acid Analysis (AAA)
Example
:Calculations Based on CHNO Analysis
Protein Assays
Enzyme-Linked Immunosorbent Assay
Gas Chromatography
DNA Hybridization
ICP/MS (AA)
Dry Weight
Microbiology Assays
Sterility
Bioburden
Endotoxin
Virus and Phage
Cell Lysis and Flocculation
Instructional Objectives
Some Elements of Cell Structure
Prokaryotic Cells
Eukaryotic Cells
Cell Lysis
Osmotic and Chemical Cell Lysis
Mechanical Methods of Lysis
Flocculation
The Electric Double Layer
Example
:Dependence of the Debye Radius on the Type of Electrolyte
Forces between Particles and Flocculation by Electrolytes
Example
:Sensitivity of Critical Flocculation Concentration to Temperature and Counter-Ion Charge Number
The Schulze-Hardy Rule

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