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| Introductory Techniques | |
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| Introduction to the Laboratory: Basic Equipment and Bacteriological Techniques | |
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| Preparation of Media and Reagents Used in Molecular Biology | |
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| Isolation of Luminescent Bacteria from Natural Sources | |
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| Restriction Digestion and Agarose Gel Electrophoresis of DNA | |
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| DNA Isolation and Analysis | |
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| Isolation of Chromosomal DNA from Vibrio fischeri | |
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| Large-Scale Purification of Plasmid DNA | |
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| Spectrophotometric Analysis of DNA | |
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| Cloning the Lux Operon | |
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| Restriction Digestion of Vibrio fischeri Genomic DNA and Plasmid Vector | |
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| Quantification of Genomic DNA by Fluorometry and Agarose Plate Fluorescence | |
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| Ligation of Restriction Fragments of Vibrio fischeri DNA to Plasmid Vector | |
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| Preparation of Competent Escherichia coli DH5 | |
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| Transformation of Competent Escherichia coli DH5 with Recombinant Plasmids | |
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| Screening the Vibrio fischeri Genomic Library for Light Producing Clones | |
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| Restriction Mapping and Southern Blotting | |
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| Small-Scale Plasmid Isolations (Mini-preps) from Bioluminescent Clones | |
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| Restriction Mapping of Plasmids from Bioluminescent Clones | |
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| Southern Blotting and Hybridization to Detect the luxA Gene | |
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| Subcloning the Lux A Gene | |
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| Restriction Digestion of lux Plasmids and Cloning Vector for Subcloning luxA | |
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| Gel Purification of DNA Restriction Fragments Containing luxA | |
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| Subcloning luxA into A Plasmid Vector | |
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| Transformation of Competent Escherichia coli DH5 with Subcloned DNA | |
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| Colony Hybridization to Screen for luxA Subclones | |
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| Small-Scale Plasmid Isolations (Mini-Preps) from luxA Clones | |
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| Advanced Techniques | |
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| Amplification of luxA from Natural Isolates by the Polymerase Chain Reaction (PCR) | |
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| Southern Blotting and Hybridization of PCR Products | |
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| DNA Sequencing of lux Genes from Plasmid Templates | |
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| Computer Analysis of DNA Sequences Using the World Wide Web | |
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| Mapping the Vibrio fischeri Genome by Pulsed Field Gel Electrophoresis | |
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| Independent Projects in Molecular Biology | |
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| Appendices | |
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| The Metric System and Units of Measure | |
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| Centrifugation | |
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| Spectrophotometry | |
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| Agarose and Polyacrylamide Gel Electrophoresis | |
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| Methylene Blue Staining of Agarose Gels | |
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| Nucleic Acid Hybridization | |
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| Alcohol Precipitation of Nucleic Acids | |
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| Care and Handling of Enzymes | |
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| Restriction Endonucleases | |
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| Enzymes Used in Molecular Biology | |
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| Maps of Cloning Vectors and Bacteriophage Lambda | |
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| Proper Handling and Disposal of Hazardous Materials | |
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| Procedures and Precautions for the Use of Radioisotopes | |
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| Equipment and Supplies | |
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| Media and Reagents | |
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| Bacterial Strains | |
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| Lists of Suppliers | |
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| Recommended References | |
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| Restriction Mapping Problems | |