Transgenic Animal Technology A Laboratory Handbook

Name: Transgenic Animal Technology A Laboratory Handbook
Price: 29.85 USD
Availability: InStock
ISBN: 9780125571661

ISBN-10: 0125571666

ISBN-13: 9780125571661

Edition: 2nd 2003 (Revised)

Authors: Carl A. Pinkert

List price: $127.00

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Description:

This update includes chapters on nuclear transfer technologies in mice, cattle, sheep and swine and on production of transgenic poultry and primates. It also contains a section on analysis, ethics and enabling technologies such as cryopreservation.

Book details

List price: $127.00
Edition: 2nd
Copyright year: 2003
Publisher: Elsevier Science & Technology
Publication date: 12/4/2002
Binding: Hardcover
Pages: 618
Size: 7.50" wide x 9.25" long x 1.00" tall
Weight: 2.376
Language: English






Overview


Introduction to transgenic animals





Transgenic animal production focusing on the mouse model


Transgenic animal production using DNA microinjection


Factors affecting transgenic animal production


Gene targeting in embryonic stem cells I: History and methodology


Gene targeting in embryonic stem cells II. Conditional technologies


Retrovirus-mediated gene transfer


Nuclear transfer technologies





Production of transgenic laboratory and domestic animal species


Production of transgenic rats


Production of transgenic rabbits


Production of transgenic fish


Production of transgenic poultry


Production of transgenic swine by DNA Microinjection


Production of transgenic ruminants by DNA Microinjection


Production of transgenic non-human primates


Retrovirus-mediated gene transfer into oocytes



Molecular biology, analyses and enabling technologies


Vector design for Transgene Expression


Analysis of transgene integration


PCR optimization for Detection of Transgenic Integration


Evaluation of Transgene Expression


Control of gene expression (from standard gain/loss of function to anti-sense to PNAs)


Enabling technologies:Cryopreservation of gametes


Enabling technologies:Embryo culture considerations


Databases, Internet Resources and Genetic Nomenclature