Molecular Biology A Project Approach

ISBN-10: 0123977207

ISBN-13: 9780123977205

Edition: 1995

Authors: Susan J. Karcher
List price: $72.95 Buy it from $3.00
eBook available
This item qualifies for FREE shipping

*A minimum purchase of $35 is required. Shipping is provided via FedEx SmartPost® and FedEx Express Saver®. Average delivery time is 1 – 5 business days, but is not guaranteed in that timeframe. Also allow 1 - 2 days for processing. Free shipping is eligible only in the continental United States and excludes Hawaii, Alaska and Puerto Rico. FedEx service marks used by permission."Marketplace" orders are not eligible for free or discounted shipping.

30 day, 100% satisfaction guarantee

If an item you ordered from TextbookRush does not meet your expectations due to an error on our part, simply fill out a return request and then return it by mail within 30 days of ordering it for a full refund of item cost.

Learn more about our returns policy

Description: This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.

New Starting from $67.40
eBooks Starting from $72.95
Buy eBooks
what's this?
Rush Rewards U
Members Receive:
coins
coins
You have reached 400 XP and carrot coins. That is the daily max!

Study Briefs

Limited time offer: Get the first one free! (?)

All the information you need in one place! Each Study Brief is a summary of one specific subject; facts, figures, and explanations to help you learn faster.

Add to cart
Study Briefs
Periodic Table Online content $4.95 $1.99
Add to cart
Study Briefs
Periodic Table Online content $4.95 $1.99

Customers also bought

Loading
Loading
Loading
Loading
Loading
Loading
Loading
Loading
Loading
Loading

Book details

List price: $72.95
Copyright year: 1995
Publisher: Elsevier Science & Technology Books
Publication date: 11/15/1995
Binding: Paperback
Pages: 280
Size: 7.75" wide x 9.25" long x 1.00" tall
Weight: 1.342
Language: English

Methods Locator
Suggested Schedule of Laboratory Protocols
Preface
Acknowledgments
Note to Users
Transposon Mutagenesis of Escherichia coli
Introduction to Transposons
Advantages of Transposon Mutagenesis
Eukaryotic Transposable Elements
Transposons and Gene Fusions
Preparing for Laboratory Exercises
Common Laboratory Rules
Guidelines for Laboratory Notebooks
Guidelines for Laboratory Reports
Using Micropipettors
Review of Sterile Technique
Tn5 Mutagenesis of Escherichia coli and Analysis of Auxotrophs: Overview
Strain List
Media Recipes
Phage [lambda] Titer
Making a Phage Stock--Growing [lambda]-Tn5'
Making Fresh Plaques
Making Phage Lysate Stocks
Transposon Mutagenesis Using [lambda]:: TnphoA'-2
Introduction to Auxotrophs
Isolation of Auxotrophs--Replica Plating, Toothpicking, or Screening on 2 EM Plates
Identification of Auxotrophs on Pool Plates
Making Pool Plates
Analysis of Auxotrophs Using a Literature Search
Genetic Mapping Strategies
References
Suggested Reading
Recombinant Dan Cloning
Introduction to Recombinant DNA Technology
Cloning Vectors
pBR322
Vectors That Yield Single-Stranded DNA
Development of the pUC Plasmids
Vectors for Cloning Large DNA Fragments
Restriction Endonucleases
Type I or Class I Restriction Endonucleases
Type II or Class II Restriction Endonucleases
Type III or Class III Restriction Endonucleases
Other Restriction Endonucleases
The Use of Restriction Endonucleases: Practical Matters
Different Restriction Endonucleases
Setting Up a Restriction Digestion
Stopping a Restriction Digestion
Ligase
Gel Electrophoresis
Structure of Agarose
Pulsed Field Gel Electrophoresis
Capillary Electrophoresis
Ethidium Bromide Staining of DNA in Gels
Ethidium Bromide Safety
Sensitivity of Detection with Ethidium Bromide
Other DNA Stains
Transformation
Background
Transformation Procedures
Recombinant DNA Cloning: Overview
Description of pUC Vectors
[beta]-Galactosidase
The Insert DNA to Be Cloned: Origin and Significance of Cosmid 203
Alternative DNAs to Clone
Recombinant DNA: P1 Level of Physical Containment--Laboratory Practices
Restriction Digestion of DNA Samples and Gel Electrophoresis of DNA Samples
Restriction Enzyme Digestion of DNA to Be Cloned
Gel Electrophoresis
Large-Scale Plasmid Isolation Using Alkaline Lysis
Determination of DNA Concentration
Recombinant DNA Cloning
Schedule for Recombinant DNA Cloning Experiment
Restriction Digestions for Cloning
Bacterial Transformation
Competent Escherichia coli Cells
Preparing and Freezing Competent Cells
Preparing Fresh Competent Escherichia coli Cells for Transformation
Using Competent Cells for Transformation
A Rapid Colony Transformation Procedure
Boiling Mini-Prep Isolation of Plasmid DNA
Alkaline Mini-Prep Procedure for Isolating Plasmid DNA
References
Suggested Reading
Southern Blot Analysis
Southern Blot Introduction
Using Southern Blot Analysis to Map Restriction Endonuclease Sites
Nonradioactive Labeling of Nucleic Acids
Horseradish Peroxidase and Enhanced Chemiluminescence
Digoxigenein Nonradioactive Labeling System
Biotin-Streptavidin Labeling System
Chromogenic Substrate for Alkaline Phosphatase
Chemiluminogenic Substrate for Alkaline Phosphatase
Autoradiography: Overview
Isolation of Nucleic Acid Fragments from Gels
Labeling Methods
Nick Translation
Oligo Labeling
Photobiotin
Hybridization to Membranes
Blot of a Dry Gel
The Attachment of Nucleic Acids to a Membrane
Southern Blot
Modifications of Standard Blotting Procedures
Mini-Southern Blotting
Bidirectional Blotting: A Sandwich Blot
Alkaline Blotting
Colony Hybridization
Isolation of DNA Fragments by Electroelution
Overview
Preparation of Dialysis Tubing
Labeling DNA to Be Used as Probes
Labeling Probe with Biotin Using Nick Translation
Separation of the Biotin-Labeled DNA from the Uncorporated Biotin-14-dATP by Exclusion Chromatography Using a Sephadex G-100 Column
Oligo Labeling of a Probe
Protobiotin Labeling of a Probe
Hybridization and Detection of Labeled Probe--A
Biotin-Labeled Nonradioactive Probe and Chromogenic Substrate
Hybridization for a Chromogenic Nonradioactive Detection System
Detection of a Biotin-Labeled Probe for a Chromogenic Nonradioactive Detection System
Hybridization and Detection of Labeled Probe--A Biotin-Labeled Nonradioactive Probe and Chemiluminogenic Substrate
Additional Notes about Nonradioactive DNA Detection Systems
Standard Southern Blot Hybridization with [superscript 32]P-Labeled Probe
References
Suggested Reading
Plant Genomic Southern Blotting With Probes for Low- and High-Copy-Number Genes
Overview of Experiment
Genomic Southern
Plant DNA Extraction Mini-Prep Procedure
"Reconstructions" for Gels
Steps of a Genomic Southern Blot
References
Suggested Reading
Rna Purification and Northern Blot Analysis
RNA Introduction: Overview of Experiment
RNA Extraction from Plant Leaves
Separating Poly(A)[superscript +] RNA from Total Cellular RNA
Batch Elution
RNA Gel: A Denaturing Formaldehyde Gel
A Northern Blot
Standard Northern Blot Hybridization Conditions for [superscript 32]P-Labeled Probe
Nonradioactive Biotin-Labeled Probes for Northern Blots
References
Suggested Reading
Polymerase Chain Reaction
Background
PCR Experiment
References
Suggested Reading
Templates for Streaking Colonies
Storing Bacterial Strains: Making Permanents
Reporter Genes
Antibiotic Information
X-gal and IPTG
More Information on Molecular Biology Protocols
Sources of Strains
List of Suppliers
Additional Information
Molecular Weight Standards
Glossary
Index
×
Free shipping on orders over $35*

*A minimum purchase of $35 is required. Shipping is provided via FedEx SmartPost® and FedEx Express Saver®. Average delivery time is 1 – 5 business days, but is not guaranteed in that timeframe. Also allow 1 - 2 days for processing. Free shipping is eligible only in the continental United States and excludes Hawaii, Alaska and Puerto Rico. FedEx service marks used by permission."Marketplace" orders are not eligible for free or discounted shipping.

Learn more about the TextbookRush Marketplace.

×