RNA Methodologies Laboratory Guide for Isolation and Characterization

ISBN-10: 0123747279

ISBN-13: 9780123747273

Edition: 4th 2009

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Description:

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. * 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques useful for all labs
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Book details

List price: $136.00
Edition: 4th
Copyright year: 2009
Publisher: Elsevier Science & Technology Books
Publication date: 9/24/2009
Binding: Comb Bound 
Pages: 744
Size: 6.50" wide x 9.00" long x 1.75" tall
Weight: 2.486
Language: English

RNA and the Cellular Biochemistry Revisited
RNA Isolation Strategies
The Truth about Tissues
Going Green: RNA and the Molecular Biology of Plants (NEW)
Isolation of Polyadenylated RNA
Quality Control for RNA Preparations (EXPANDED)
Resilient Ribonucleases
Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED)
Electrophoresis of RNA (EXPANDED)
Photodocumentation and Image Analysis (EXPANDED)
Northern Analysis
Nucleic Acid Probe Technology
Practical Nucleic Acid Hybridization
Principles of Detection (EXPANDED)
Quantification of Specific mRNAs by Nuclease Protection
Analysis of Nuclear RNA (EXPANDED)
cDNA Synthesis (EXPANDED)
RT-PCR (NEW)
Quantitative PCR Techniques
Functional Genomics and Transcript Profiling (NEW)
High-Throughput Analysis of Gene Expression
Non-Array Methods for Global Analysis of Gene Expression (NEW)
RNA Interference: Targeted Gene Silencing (EXPANDED)
Genomes, Transcriptomes, Proteomes, and Bioinformatics
An RNA Paradigm
Maintaining Complete and Accurate Records
Converting Mass to Moles (NEW)
Useful Stock Solutions for the Molecular Biologist
Phenol Preparation
Disposal of Ethidium Bromide and SYBR Green Solutions
DNase I Removal of DNA From An RNA Sample
DNase Incubation to Remove RNA from a DNA Sample
Deionization of Formamide, Formaldehyde, and Glyoxal
Silanizing Centrifuge Tubes and Glassware
Trypsinization Protocol for Anchorage-Dependent Cells
Isolation of High-Molecular-Weight DNA by Salting-Out
Electrophoresis: Principles, Parameters, and Safety
Polyacrylamide Gel Electrophoresis
Dot Blot Analysis (NEW)
Centrifugation as a Mainstream Tool for the Molecular Biologist
Selected Suppliers of Equipment, Reagents, and Services
Useful SI Units
Common Abbreviations
Trade Citations
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