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Lipid Modifications of Proteins

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ISBN-10: 012182151X

ISBN-13: 9780121821517

Edition: 1995

Authors: John N. Abelson, Melvin I. Simon, Patrick J. Casey, Janice E. Buss

List price: $72.95
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Description:

General Description of the Volume: This volume presents the best techniques and strategies for the study of lipid modified proteins, with particular emphasis on methods which evaluate the functional significance or biological impact of lipid modification. Included are the biological methods for the study of function (yeast genetics; cloning strategies; mutational analysis; expression systems), biochemical methods for the study and purification of enzymes or modified proteins (in vitro assays using peptide, native, or recombinant protein substrates; coupled in vitro cDNA transcription, translation/modification; bacolovirus expression; lipid analogs/inhibitors); physical methods for the…    
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Book details

List price: $72.95
Copyright year: 1995
Publisher: Elsevier Science & Technology
Publication date: 6/23/1995
Binding: Hardcover
Pages: 754
Size: 5.98" wide x 9.02" long x 0.61" tall
Weight: 2.574

Prenylation
Bacterial Expression and Purification of Human Protein Prenyltransferases Using Epitope-Tagged, Translationally Coupled Systems
Isolation of Protein Prenyltransferases from Bovine Brain and Baculovirus Expression System
Substrate Interactions of Protein Prenyltransferases
Continuous Fluorescence Assay for Protein Prenyltransferases
In Vivo Assays for Farnesyltransferase Inhibitors with Saccharomyces cerevisiae
Mutagenesis and Biochemical Analysis of Recombinant Yeast Prenyltransferases
Characterization of Protein Prenylation in Saccharomyces cerevisiae
Coupled Translation/Prenylation of Rab Proteins in Vitro
Prenylation and Carboxylmethylation of G-Protein gamma Subunit
Mutation and Analysis of Prenylation Signal Sequences
Effects of Prenyl Modifications on Interactions of Small G Proteins with Regulators
Expression Systems for Nuclear Lamin Proteins: Farnesylation in Assembly of Nuclear Lamina
Prenylation-Dependent Targeting of G-Protein-Coupled Receptor Kinases
Prenylated Peptides in Identification of Specific Binding Proteins
Lipid-Mediated a-Factor Interactions with Artificial Membranes
Prenylation-Dependent Processing
Synthetic Prenylated Peptides: Studying Prenyl Protein-Specific Endoprotease and Other Aspects of Protein Prenylation
Inhibitors of Prenylated ProteinEndoprotease
Prenylcysteine Analogs to Study Function of Carboxylmethylation in Signal Transduction
Farnesylcysteine Analogs to Probe Role of Prenylated Protein Methyltransferase
Ras and a-Factor Converting Enzyme
Yeast STE14 Methyltransferase, Expressed as TrpE-STE14 Fusion Protein in Escherichia coli, for in Vitro Carboxylmethylation of Prenylated Polypeptides
Palmitoylation
Analysis of Ras Acylation Sites: Mutagenesis and Transfection
Inhibition of Dynamic Protein Palmitoylation in Intact Cells with Tunicamycin
Dynamic Palmitoylation of G-Protein-Coupled Receptors in Eukaryotic Cells
Palmitoylation of G-Protein ( Subunits
Detecting Radiolabeled Lipid-Modified Proteins in Polyacrylamide Gels
Assay and Isolation of Palmitoyl-Protein Thioesterase from Bovine Brain Using Palmitoylated H-Ras as Substrate