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Arachidonate Related Lipid Mediators

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ISBN-10: 0121820882

ISBN-13: 9780121820886

Edition: 1990

Authors: John N. Abelson, Melvin I. Simon, Robert C. Murphy, Frank A. Fitzpatrick

List price: $72.95
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Description:

This volume includes information on arachidonic acid metabolites and platelet activating factor. Assays for the quantitative measurement of prostaglandins, leukotrienes, and platelet-activating factor, including enzyme immunoassays and mass spectrophotometric techniques, are presented. Preparation and analysis of cytochrome P-450 metabolites of arachidonic acid are discussed. The isolation, purification, and assay of enzymes important in the biosynthesis and metabolism of these lipids are included.
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Book details

List price: $72.95
Copyright year: 1990
Publisher: Elsevier Science & Technology
Publication date: 9/21/1990
Binding: Hardcover
Pages: 685
Size: 5.94" wide x 9.00" long
Weight: 2.904

Robert C Murphy graduated from Massachusetts Institute of Technology with a PhD in chemistry and he is currently a University Distinguished Professor at the University of Colorado. He has worked in the area of mass spectrometry and eicosanoid biochemistry for approximately 40 years with much of his research activities centered on the use of mass spectrometry to studies of arachidonic acid biochemistry and formation of the biologically active leukotriene mediators. His interests also include the structural characterizaton of bioactive lipid products derived from the reaction of reactive oxygen species with cellular lipids. Over 400 peer-reviewed papers in scientific journals and several…    

Nomenclature
Assays: Prostaglandins
Electron-Capture Negative-Ion Chemical Ionization Mass Spectrometry of Lipid Mediators
Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase
Radioimmunoassay of 11-Dehydrothromboxane B2
Measurement of Thromboxane Metabolites by Gas Chromatography-Mass Spectrometry
Quantification of Prostaglandin F2 Stable Isotope Dilution Mass Spectrometric Assay
Immunoaffinity Purification-Chromatographic Quantitative Analysis of Arachidonic Acid Metabolites
Leukotrienes
Preparation of Tetradeuterated Leukotriene A4 Methyl Ester: Methyl-[11,12,14,15-2H4]-(5S,6S)-Oxide-(7E,9E,11Z,14Z)-Eicosatetraenoate
Quantitative Gas Chromatography-Mass Spectrometry Analysis of Leukotriene B4
Enzyme Immunoassays for Leukotrienes C4 and E4 Using Acetylcholinesterase
Quantitation of Sulfidopeptide Leukotrienes in Biological Fluids by Gas Chromatography-Mass Spectrometry
Automated On-Line Extraction and Profiling of Lipoxygenase Products of Arachidonic Acid by High-Performance Liquid Chromatography
Preparation of Antibodies Directed against Leukotrienes
Platelet-Activating Factor
Bioassay of Platelet-Activating Factor Acether by Rabbit Platelet Aggregation
Bioassay of Platelet-Activating Factor by Release of [3H]Serotonin
Quantitation of Platelet-Activating Factor by Gas Chromatography-Mass Spectrometry
Quantitative Analysis of Platelet-Activating Factor by Gas Chromatography-Negative-Ion Chemical Ionization Mass Spectrometry
Isolation of Platelet-Activating Factor and Purification by Thin-Layer Chromatography
Separation and Characterization of Arachidonate-Containing Phosphoglycerides
Other Oxidative Products
High-Performance Liquid Chromatography Separation and Determination of Lipoxins
Quantitation of Epoxy- and Dihydroxyeicosatrienoic Acids by Stable Isotope-Dilution Mass Spectrometry
High-Performance Liquid Chromatography for Chiral Analysis of Eicosanoids
Phospholipase A2 and Phospholipids
Extraction of Phospholipids and Analysis of Phospholipid Molecular Species
Macrophage Phospholipase A2 Specific for sn-2-Arachidonic Acid
Measurement of Phosphoinositide-Specific Phospholipase C Activity
Solubilization of Arachidonate CoA Ligase from Cell Membranes, Chromatographic Separation from Nonspecific Long-Chain-Fatty-Acid-CoA