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Preface | |
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Laboratory Safety | |
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Laboratory Safety Agreement | |
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Basic Microbiology | |
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Introduction to Microbiology | |
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Ubiquity of Microorganisms | |
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Pure Culture and Aseptic Technique | |
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Introduction to the Compound Light Microscope | |
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The Oil Immersion Lens | |
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Introduction to Staining of Microorganisms | |
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Simple Stains: Positive and Negative-Stains | |
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Differential and Other Special Stains | |
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Introduction to Microbial Growth | |
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Chemically Defined, Complex, Selective, and Differential Media | |
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Quantification of Microorganisms | |
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Introduction to the Environment and Microbial Growth | |
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Aerobic and Anaerobic Growth | |
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The Effect of Incubation Temperature on Generation Time | |
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Introduction to Control of Microbial Growth | |
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Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time | |
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Control of Microbial Growth with Ultraviolet Light | |
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Changes in Osmotic Pressure Due to Salt and Sugar and Their Effects on Microbial Growth | |
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Antibiotics | |
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Antiseptics and Disinfectants | |
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Introduction to Microbial Genetics | |
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Selection of Bacterial Mutants Resistant to Antibiotics | |
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Transformation: A Form of Genetic Recombination | |
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Gene Regulation: Induction and Catabolite Repression | |
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The Other Microbial World | |
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Introduction to the Other Microbial World | |
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Microscopic Identification of Fungi | |
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Parasitology: Protozoa and Helmindis | |
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Titering Prokaryotic Viruses | |
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Microbes and Humans | |
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Introduction to Medical Microbiology | |
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Normal Skin Biota | |
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Streptococci and Respiratory Microorganisms | |
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Identification of Enteric Gram-Negative Rods | |
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Clinical Unknown Identification | |
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Immunology | |
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Introduction to Some Immunological Principles and Techniques | |
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Differential White Blood Cell Stains | |
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Lysozyme, an Enzyme Contributing to Innate Immunity | |
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Enzyme-Linked Immunosorbent Assay (ELISA) | |
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Public Health | |
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Introduction to the Prevention and Control of Communicable Diseases | |
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Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques | |
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Epidemiology | |
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Applications of Biotechnology | |
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Introduction to Biotechnology | |
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Identifying DNA with Restriction Enzymes and the Polymerase Chain Reaction (PCR) | |
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Electrophoresis | |
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Identification of Bacteria Using the Ribosomal Database Project | |
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Microarray | |
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Individual Projects | |
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Introduction to the Individual Projects | |
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Hydrocarbon-Degrading Bacteria: Cleaning Up after Oil Spills | |
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Luminescent Bacteria: Bacteria That Produce Light | |
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Appendices | |
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Culture Media and Reagent Formulas | |
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Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in This Manual | |
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Dilution Practice Problems | |
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Alternative Procedures | |
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Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size | |
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History and Working Principles of Light Microscopy | |
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Index | |