Electron Microscopy in Biology
Electron Microscopy: A Practical Approach published in 1987, dealt specifically with nucleic acids and proteins. This volume provides detailed protocols for a wide range of procedures relating to the electron microscopy of tissues, cells, and their More...
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Publisher: Oxford University Press, Incorporated
Binding: Comb Bound
Size: 7.00" wide x 9.63" long x 0.93" tall
Electron Microscopy: A Practical Approach published in 1987, dealt specifically with nucleic acids and proteins. This volume provides detailed protocols for a wide range of procedures relating to the electron microscopy of tissues, cells, and their components with the emphasis on biomedical applications. The fundamental methods for specimen preparation, tissue fixation, embedding, and sectioning are covered in depth and are followed by techniques in freeze-substitution, immunogold labelling, autoradiography, and enzyme histochemistry. Although the technical details in the book relate mainly to tissues and cells, many of the procedures can be adapted for isolated organelles and membrane fractions, viral particles, macromolecular assemblies, and large proteins. This is a broadly based compilation of the most important specimen preparation techniques and their applications. It will be of interest to researchers and advanced students working in almost every biomedical discipline, plus technicians in electron microscopy units.
|Embedding and Thin Section Preparations|
|Freeze-substitution and Other Low Temperature Embedding Methods|
|Immunogold Labelling Technique|
|Electron Microscopic Autoradiographic Techniques|
|Electron Microscopical Enzyme Histochemistry|
|High Resolution Shadowing|
|Transmission Electron Microscopy of Vitrified Biological Macromolecular Assemblies|
|Quantitative Biological X-ray Microanalysis|
|Three-Dimensional Electron Microscopy of Tissues and Cells|
|Table of Contents provided by Publisher. All Rights Reserved.|