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    Electron Microscopy in Biology

    ISBN-10: 0199632197
    ISBN-13: 9780199632190
    Author(s): Robert Harris
    Description: Electron Microscopy: A Practical Approach published in 1987, dealt specifically with nucleic acids and proteins. This volume provides detailed protocols for a wide range of procedures relating to the electron microscopy of tissues, cells, and their  More...
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    Publisher: Oxford University Press, Incorporated
    Binding: Comb Bound 
    Pages: 328
    Size: 7.00" wide x 9.63" long x 0.93" tall
    Weight: 1.606
    Language: English

    Electron Microscopy: A Practical Approach published in 1987, dealt specifically with nucleic acids and proteins. This volume provides detailed protocols for a wide range of procedures relating to the electron microscopy of tissues, cells, and their components with the emphasis on biomedical applications. The fundamental methods for specimen preparation, tissue fixation, embedding, and sectioning are covered in depth and are followed by techniques in freeze-substitution, immunogold labelling, autoradiography, and enzyme histochemistry. Although the technical details in the book relate mainly to tissues and cells, many of the procedures can be adapted for isolated organelles and membrane fractions, viral particles, macromolecular assemblies, and large proteins. This is a broadly based compilation of the most important specimen preparation techniques and their applications. It will be of interest to researchers and advanced students working in almost every biomedical discipline, plus technicians in electron microscopy units.

    Fixation
    Embedding and Thin Section Preparations
    Freeze-substitution and Other Low Temperature Embedding Methods
    Immunogold Labelling Technique
    Electron Microscopic Autoradiographic Techniques
    Electron Microscopical Enzyme Histochemistry
    High Resolution Shadowing
    Freeze-fracture Techniques
    Negative Staining
    Transmission Electron Microscopy of Vitrified Biological Macromolecular Assemblies
    Quantitative Biological X-ray Microanalysis
    Three-Dimensional Electron Microscopy of Tissues and Cells
    Table of Contents provided by Publisher. All Rights Reserved.

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